Figure 3. Potentiation is CGRP-dependent and influences presynaptic release probability in females.

(A) Potentiation of PB_CGRP glutamate signaling in female neurons is reproducible, as a second PB CGRP release event (CGRP₂) induces a similar level of potentiation as an initial PB CGRP release event (CGRP₁). (B) This potentiation is CGRP-dependent as evidenced by its suppression by 1 μM CGRP8–37, CGRP receptor antagonist. (C) The potentiation is not associated in with a change in paired pulse ratios, indicating that it does not involve presynaptic mechanisms. Black dashed lines indicate group averages. Inset shows traces of paired pulses (100 msec interstimulus interval) at baseline (black) and after high frequency optic tetanus (red).