Table 1. Primer sequences used.
| gene | forward sequence | reverse sequence |
|---|---|---|
| DUSP6 | AACCTGTCCCAGTTTTTCCCT | GCCAAGCAATGTACCAAGACAC |
| EN2 | TCTACTGTACGCGCTACTCG | CGCTTGTCCTCTTTGTTCGG |
| ETV1 | CCAATAGTCAGCGTGGGAGAA | TTCTGCAAGCCATGTTTCCTGT |
| ETV4 | TCAAACAGGAACAGACGGACTT | AGGTTTCTCATAGCCATAGCCCA |
| ETV5 | ACACGGGTTCCAGTCACCAA | GCTGCTGGAGAAATAACCCCC |
| FOXG1 | GCCAAGTTTTACGACGGGAC | AAGGGTTGGAAGAAGACCCC |
| GAPDH | CGTGGAAGGACTCATGACCA | CAGTCTTCTGGGTGGCAGTGA |
| NANOG | CAAAGGCAAACAACCCACTT | TCTGCTGGAGGCTGAGGTAT |
| NR2F1 | TGGCAATGGTAGTTAGCAGCT | TTGAGGCACTTCTTGAGGCG |
| OCT4 | GTGGAGGAAGCTGACAACAA | ATTCTCCAGGTTGCCTCTCA |
| OTX2 | CCTCACTCGCCACATCTACT | CTTGGTGGGTGGGTTTGGAG |
| PAX6 | AGTGAATCAGCTCGGTGGTGTCTT | TGCAGAATTCGGGAAATGTCGCAC |
| SIX3 | CAGCAAGAAACGCGAACTGG | AATGGCCTGGTGCTGGA |
| SOX2 | AGGATAAGTACACGCTGCCC | TAACTGTCCATGCGCTGGTT |
| SPRY4 | CGGCTTCAGGATTTACACAGAC | CTGCAAACCGCTCAATACAGG |
| β-Actin | CTTCGCGGGCGACGAT | ACATAGGAATCCTCCTGACCC |