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. 1982 Mar 15;202(3):707–716. doi: 10.1042/bj2020707

Interactions between platelet-surface glycoproteins. Identification of glycoproteins capable of binding to platelet surfaces.

D J Bowles, C Brunton
PMCID: PMC1158166  PMID: 7092840

Abstract

1. Platelets have been isolated from plasma and their surface glycoconjugates radioactively-labelled using galactose oxidase and NaB3H4. 2. Conditions have been defined for optimal labelling of glycoproteins and a membrane fraction enriched in plasma membrane has been prepared and characterized by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. 3. Desialylated glycoproteins that act as receptors to peanut agglutinin and lentil lectin have been purified from a detergent extract of plasma membrane. 4. Two glycosylated polypeptides that are able to bind to the surfaces of platelets have been identified and some characteristics of the binding have been investigated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson L. C., Gahmberg C. G. Surface glycoproteins of human white blood cells. Analysis by surface labeling. Blood. 1978 Jul;52(1):57–67. [PubMed] [Google Scholar]
  2. Baumgartner H. R. The role of blood flow in platelet adhesion, fibrin deposition, and formation of mural thrombi. Microvasc Res. 1973 Mar;5(2):167–179. doi: 10.1016/0026-2862(73)90069-1. [DOI] [PubMed] [Google Scholar]
  3. Bowles D. J. Lectins as membrane components: implications of lectin--receptor interaction. FEBS Lett. 1979 Jun 1;102(1):1–3. doi: 10.1016/0014-5793(79)80915-1. [DOI] [PubMed] [Google Scholar]
  4. Clemetson K. J., Pfueller S. L., Luscher E. F., Jenkins C. S. Isolation of the membrane glycoproteins of human blood platelets by lectin affinity chromatography. Biochim Biophys Acta. 1977 Feb 4;464(3):493–508. doi: 10.1016/0005-2736(77)90025-6. [DOI] [PubMed] [Google Scholar]
  5. Cooper H. A., Clemetson K. J., Lüscher E. F. Human platelet membrane receptor for bovine von Willebrand factor (platelet aggregating factor): an integral membrane glycoprotein. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1069–1073. doi: 10.1073/pnas.76.3.1069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cross G. A. Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Parasitology. 1975 Dec;71(3):393–417. doi: 10.1017/s003118200004717x. [DOI] [PubMed] [Google Scholar]
  7. Entlicher G., Tichá M., Kostír J. V., Kocourek J. Studies on phytohemagglutinins. II. Phytohemagglutinins of Pisum sativum L. and Lens esculenta Moench: specific interactions with carbohydrates. Experientia. 1969 Jan 15;25(1):17–19. doi: 10.1007/BF01903864. [DOI] [PubMed] [Google Scholar]
  8. Gartner T. K., Gerrard J. M., White J. G., Williams D. C. Fibrinogen is the receptor for the endogenous lectin of human platelets. Nature. 1981 Feb 19;289(5799):688–690. doi: 10.1038/289688a0. [DOI] [PubMed] [Google Scholar]
  9. Gartner T. K., Phillips D. R., Williams D. C. Expression of thrombin-enhanced platelet lectin activity is controlled by secretion. FEBS Lett. 1980 May 5;113(2):196–199. doi: 10.1016/0014-5793(80)80590-4. [DOI] [PubMed] [Google Scholar]
  10. George J. N., Lyons R. M., Morgan R. K. Membrane changes associated with platelet activation. Exposure of actin on the platelet surface after thrombin-induced secretion. J Clin Invest. 1980 Jul;66(1):1–9. doi: 10.1172/JCI109821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. George J. N. Studies on platelet plasma membranes. IV. Quantitative analysis of platelet membrane glycoproteins by (125I)-diazotized diiodosulfanilic acid labeling and SDS-polyacrylamide gel electrophoresis. J Lab Clin Med. 1978 Sep;92(3):430–446. [PubMed] [Google Scholar]
  12. George S. G., Kenny J. Studies on the enzymology of purified preparations of brush border from rabbit kidney. Biochem J. 1973 May;134(1):43–57. doi: 10.1042/bj1340043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Greenaway P. J., LeVine D. Binding of N-acetyl-neuraminic acid by wheat-germ agglutinin. Nat New Biol. 1973 Feb 7;241(110):191–192. doi: 10.1038/newbio241191a0. [DOI] [PubMed] [Google Scholar]
  14. Hagen I., Nurden A., Bjerrum O. J., Solum N. O., Caen J. Immunochemical evidence for protein abnormalities in platelets from patients with Glanzmann's thrombasthenia and Bernard-Soulier syndrome. J Clin Invest. 1980 Mar;65(3):722–731. doi: 10.1172/JCI109719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hatton M. W., Regoeczi E. A simple method for the purification of commercial neuraminidase preparations free from proteases. Biochim Biophys Acta. 1973 Nov 15;327(1):114–120. doi: 10.1016/0005-2744(73)90108-3. [DOI] [PubMed] [Google Scholar]
  16. Kinoshita T., Nachman R. L., Minick R. Isolation of human platelet plasma membranes with polylysine beads. J Cell Biol. 1979 Sep;82(3):688–696. doi: 10.1083/jcb.82.3.688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Lotan R., Skutelsky E., Danon D., Sharon N. The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea). J Biol Chem. 1975 Nov 10;250(21):8518–8523. [PubMed] [Google Scholar]
  19. Marguerie G. A., Plow E. F., Edgington T. S. Human platelets possess an inducible and saturable receptor specific for fibrinogen. J Biol Chem. 1979 Jun 25;254(12):5357–5363. [PubMed] [Google Scholar]
  20. Mosher D. F., Vaheri A., Choate J. J., Gahmberg C. G. Action of thrombin on surface glycoproteins of human platelets. Blood. 1979 Mar;53(3):437–445. [PubMed] [Google Scholar]
  21. Nachman R. L., Kinoshita T., Ferris B. Structural analysis of human platelet membrane glycoprotein I complex. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2952–2954. doi: 10.1073/pnas.76.6.2952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Niewiarowski S., Budzynski A. Z., Morinelli T. A., Brudzynski T. M., Stewart G. J. Exposure of fibrinogen receptor on human platelets by proteolytic enzymes. J Biol Chem. 1981 Jan 25;256(2):917–925. [PubMed] [Google Scholar]
  23. Okumura I., Lombart C., Jamieson G. A. Platelet glycocalicin. II. Purification and characterization. J Biol Chem. 1976 Oct 10;251(19):5950–5955. [PubMed] [Google Scholar]
  24. Phillips D. R., Agin P. P. Platelet plasma membrane glycoproteins. Evidence for the presence of nonequivalent disulfide bonds using nonreduced-reduced two-dimensional gel electrophoresis. J Biol Chem. 1977 Mar 25;252(6):2121–2126. [PubMed] [Google Scholar]
  25. Phillips D. R., Agin P. P. Platelet plasma membrane glycoproteins. Identification of a proteolytic substrate for thrombin. Biochem Biophys Res Commun. 1977 Apr 25;75(4):940–947. doi: 10.1016/0006-291x(77)91473-5. [DOI] [PubMed] [Google Scholar]
  26. Phillips D. R., Jakábová M. Ca2+-dependent protease in human platelets. Specific cleavage of platelet polypeptides in the presence of added Ca2+. J Biol Chem. 1977 Aug 25;252(16):5602–5605. [PubMed] [Google Scholar]
  27. Phillips D. R., Jennings L. K., Edwards H. H. Identification of membrane proteins mediating the interaction of human platelets. J Cell Biol. 1980 Jul;86(1):77–86. doi: 10.1083/jcb.86.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sedmak J. J., Grossberg S. E. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. Anal Biochem. 1977 May 1;79(1-2):544–552. doi: 10.1016/0003-2697(77)90428-6. [DOI] [PubMed] [Google Scholar]

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