Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1982 Apr 1;203(1):323–326. doi: 10.1042/bj2030323

Difference in thermal stability of type-I and type-III collagen from rat skin

Carl Christian Danielsen 1
PMCID: PMC1158226  PMID: 7103945

Abstract

Type-I and type-III collagens were obtained by differential salt fractionation of neutral-salt-soluble collagen from rat skin. Their thermal stabilities were determined by u.v. difference spectroscopy. The `melting' temperature (Tm) in 5mm-acetic acid of type-III collagen was almost 2°C above that of type-I collagen. Intramolecular covalent cross-linking had no effect on the thermal stability.

Full text

PDF
323

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey A. J., Robins S. P. Current topics in the biosynthesis, structure and function of collagen. Sci Prog. 1976 Autumn;63(251):419–444. [PubMed] [Google Scholar]
  2. Burjanadze T. V. Hydroxyproline content and location in relation to collagen thermal stability. Biopolymers. 1979 Apr;18(4):931–938. doi: 10.1002/bip.1979.360180413. [DOI] [PubMed] [Google Scholar]
  3. Burke J. M., Balian G., Ross R., Bornstein P. Synthesis of types I and III procollagen and collagen by monkey aortic smooth muscle cells in vitro. Biochemistry. 1977 Jul 12;16(14):3243–3249. doi: 10.1021/bi00633a031. [DOI] [PubMed] [Google Scholar]
  4. Byers P. H., McKenney K. H., Lichtenstein J. R., Martin G. R. Preparation of type III procollagen and collagen from rat skin. Biochemistry. 1974 Dec 3;13(25):5243–5248. doi: 10.1021/bi00722a030. [DOI] [PubMed] [Google Scholar]
  5. Chung E., Miller E. J. Collagen polymorphism: characterization of molecules with the chain composition (alpha 1 (3)03 in human tissues. Science. 1974 Mar;183(130):1200–1201. doi: 10.1126/science.183.4130.1200. [DOI] [PubMed] [Google Scholar]
  6. Danielsen C. C. Mechanical properties of reconstituted collagen fibrils. A study on reconstitution methodology and influence of in vitro maturation. Connect Tissue Res. 1981;9(1):51–57. doi: 10.3109/03008208109160239. [DOI] [PubMed] [Google Scholar]
  7. Fujii T., Kühn K. Isolation and characterization of pepsin-treated type III collagen from calf skin. Hoppe Seylers Z Physiol Chem. 1975 Nov;356(11):1793–1801. doi: 10.1515/bchm2.1975.356.2.1793. [DOI] [PubMed] [Google Scholar]
  8. Furthmayr H., Timpl R. Characterization of collagen peptides by sodium dodecylsulfate-polyacrylamide electrophoresis. Anal Biochem. 1971 Jun;41(2):510–516. doi: 10.1016/0003-2697(71)90173-4. [DOI] [PubMed] [Google Scholar]
  9. Herrmann H., Dessau W., Fessler L. I., von der Mark K. Synthesis of types I, III and AB2 collagen by chick tendon fibroblasts in vitro. Eur J Biochem. 1980 Mar;105(1):63–74. doi: 10.1111/j.1432-1033.1980.tb04474.x. [DOI] [PubMed] [Google Scholar]
  10. Peltonen L., Palotie A., Hayashi T., Prockop D. J. Thermal stability of type I and type III procollagens from normal human fibroblasts and from a patient with osteogenesis imperfecta. Proc Natl Acad Sci U S A. 1980 Jan;77(1):162–166. doi: 10.1073/pnas.77.1.162. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES