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. 1982 Apr 15;204(1):221–227. doi: 10.1042/bj2040221

A serum protein inhibitor of acid lipase and its possible role in lipid accumulation in cultured fibroblasts.

E Gorin, H Gonen, S Dickbuch
PMCID: PMC1158336  PMID: 7115323

Abstract

Histochemical examination of L929 fibroblasts indicates massive accumulation of intracellular lipids in cells grown in medium supplemented with 10% calf serum. The present study suggests that the accumulation of triacylglycerols in these cells may be due to the inhibition of acid lipase activity by a serum component present in the culture medium. This is based on the following observations. (a) Acid lipase appears to be the major intracellular enzyme responsible for triacylglycerol catabolism in L929 cells. (b) The acid lipase is strongly inhibited by either human of calf serum. Several lines of evidence show that the inhibitor is a serum protein: it is heat-labile, non-dialysable and is destroyed by trypsin. It is present mainly in Cohn's fraction IV and has mol.wt. approx. 50000. (c) Lipid accumulation in intact cells is reduced when cells are grown on a limited supply of serum (2%) and is elevated by the addition of Cohn's fraction IV, freed of lipoproteins, to the growth medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adebonojo F. O., Cortner J. A., Coates P. M. Neutral and acid lipase of mouse 3T3 fibroblasts with increased adipose conversion. Cell Differ. 1977 Dec;6(5-6):307–312. doi: 10.1016/0045-6039(77)90005-7. [DOI] [PubMed] [Google Scholar]
  2. Bailey J. M., Howard B. V., Tillman S. F. Lipid metabolism in cultured cells. XI. Utilization of serum triglycerides. J Biol Chem. 1973 Feb 25;248(4):1240–1247. [PubMed] [Google Scholar]
  3. Beaudet A. L., Lipson M. H., Ferry G. D., Nichols B. L., Jr Acid lipase in cultured fibroblasts: cholesterol ester storage disease. J Lab Clin Med. 1974 Jul;84(1):54–61. [PubMed] [Google Scholar]
  4. Belfrage P., Vaughan M. Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides. J Lipid Res. 1969 May;10(3):341–344. [PubMed] [Google Scholar]
  5. Ciehanover A., Hod Y., Hershko A. A heat-stable polypeptide component of an ATP-dependent proteolytic system from reticulocytes. Biochem Biophys Res Commun. 1978 Apr 28;81(4):1100–1105. doi: 10.1016/0006-291x(78)91249-4. [DOI] [PubMed] [Google Scholar]
  6. Cortner J. A., Coates P. M., Swoboda E., Schnatz J. D. Genetic variation of lysosomal acid lipase. Pediatr Res. 1976 Nov;10(11):927–932. doi: 10.1203/00006450-197611000-00005. [DOI] [PubMed] [Google Scholar]
  7. DOLE V. P., MEINERTZ H. Microdetermination of long-chain fatty acids in plasma and tissues. J Biol Chem. 1960 Sep;235:2595–2599. [PubMed] [Google Scholar]
  8. Geyer R. P. Uptake and retention of fatty acids by tissue culture cells. Wistar Inst Symp Monogr. 1967;6:33–47. [PubMed] [Google Scholar]
  9. Goldstein J. L., Brown M. S. The low-density lipoprotein pathway and its relation to atherosclerosis. Annu Rev Biochem. 1977;46:897–930. doi: 10.1146/annurev.bi.46.070177.004341. [DOI] [PubMed] [Google Scholar]
  10. HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HOKIN L. E., HOKIN M. R. The mechanism of phosphate exchange in phosphatidic acid in response to acetylcholine. J Biol Chem. 1959 Jun;234(6):1387–1390. [PubMed] [Google Scholar]
  12. Hall C. W., Cantz M., Neufeld E. F. A -glucuronidase deficiency mucopolysaccharidosis: studies in cultured fibroblasts. Arch Biochem Biophys. 1973 Mar;155(1):32–38. doi: 10.1016/s0003-9861(73)80006-2. [DOI] [PubMed] [Google Scholar]
  13. Hayase K., Tappel A. L. Specificity and other properties of lysosomal lipase of rat liver. J Biol Chem. 1970 Jan 10;245(1):169–175. [PubMed] [Google Scholar]
  14. Klintworth G. K., Hijmans J. C. The induction by serum of lipid storage in cells of the cornea grown in culture. Am J Pathol. 1970 Mar;58(3):403–418. [PMC free article] [PubMed] [Google Scholar]
  15. Kyriakides E. C., Paul B., Balint J. A. Lipid accumulation and acid lipase deficiency in fibroblasts from a family with Wolman's disease, and their apparent correction in vitro. J Lab Clin Med. 1972 Dec;80(6):810–816. [PubMed] [Google Scholar]
  16. Lengle E., Geyer R. P. Strain L fibroblast lipases. Purification and properties. Biochim Biophys Acta. 1973 Feb 14;296(2):411–425. doi: 10.1016/0005-2760(73)90099-4. [DOI] [PubMed] [Google Scholar]
  17. Mackenzie C. G., Mackenzie J. B., Reiss O. K. Regulation of cell lipid metabolism and accumulation. V. Quantitative and structural aspects of triglyceride accumulation caused by lipogenic substances. Wistar Inst Symp Monogr. 1967;6:63–83. [PubMed] [Google Scholar]
  18. Mahadevan S., Tappel A. L. Lysosomal lipases of rat liver and kidney. J Biol Chem. 1968 Jun 10;243(11):2849–2854. [PubMed] [Google Scholar]
  19. Moskowitz M. S. Fatty acid-induced steatosis in monolayer cell cultures. Wistar Inst Symp Monogr. 1967;6:49–62. [PubMed] [Google Scholar]
  20. Nilsson-Ehle P., Schotz M. C. A stable, radioactive substrate emulsion for assay of lipoprotein lipase. J Lipid Res. 1976 Sep;17(5):536–541. [PubMed] [Google Scholar]
  21. Rothblat G. H., Arbogast L. Y., Ouellette L., Howard B. V. Preparation of delipidized serum protein for use in cell culture systems. In Vitro. 1976 Aug;12(8):554–557. doi: 10.1007/BF02797438. [DOI] [PubMed] [Google Scholar]

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