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. 1982 Apr 15;204(1):307–312. doi: 10.1042/bj2040307

Effect of 4-hydroxypyrazole on tryptophan and formate metabolism in isolated rat liver cells.

J S Cook, C I Pogson
PMCID: PMC1158346  PMID: 6810879

Abstract

1. 4-Hydroxypyrazole inhibits flux through tryptophan 2.3-dioxygenase in cells. The inhibition is apparently non-competitive with Ki = 0.15 mM. 2. Hydroxypyrazole inhibits the oxidation of formate to CO2 in liver cells. 3. Glycollate, which generates H2O2, stimulates formate oxidation. This process is inhibited by 4-hydroxypyrazole. 4. Methionine stimulates formate oxidation in cells and this stimulation is insensitive to 4-hydroxypyrazole. 5. It is concluded that, in freshly isolated liver cells, formate oxidation proceeds by a pathway involving catalase. In vivo, or when methionine is added to cell incubations, the pathway of oxidation involves tetrahydrofolate, and is insensitive to catalase inhibitors. 6. Methionine at physiological concentrations inhibits the activity of tryptophan 2,3-dioxygenase in isolated liver cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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