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. 1982 May 15;204(2):393–398. doi: 10.1042/bj2040393

Tissue and whole-body protein synthesis in immature Zucker rats and their relationship to protein deposition.

P J Reeds, P Haggarty, K W Wahle, J M Fletcher
PMCID: PMC1158364  PMID: 7115335

Abstract

The rates of protein synthesis in skeletal muscle, intestine, liver and in the whole body of immature (18 and 25 days old) lean and obese male Zucker rats were measured. In addition, the rate of deposition of whole-body and skeletal-muscle protein over the period 16-27 days post partum was measured by comparative slaughter and analysis of the composition of the body. At 16 days post partum, lean and obese rats had similar body protein contents, but thereafter the rate of protein deposition in the body and skeletal-muscle mass was decreased in the obese rats. The decrease was particularly marked before 21 days of age, and between 23 and 27 days post partum the fractional rate of protein deposition was the same in lean and obese rats. Of the tissues that were studied, only skeletal muscle had a lower fractional rate of protein synthesis in the obese rats. At 18 days post partum, the decrease in the absolute rate of protein synthesis in skeletal muscle accounted for at least 80% of the decline in protein synthesis in the whole body. After weaning, phenotypic differences in protein synthesis was less marked than at 18 days of age, and skeletal muscle accounted for only 50% of the difference in body protein synthesis between phenotypes. The possibility that a change in the function of the adrenal cortex contributes to differences in protein metabolism between lean and obese Zucker rats is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen C., Kendall J. W. Maturation of the circadian rhythm of plasma corticosterone in the rat. Endocrinology. 1967 May;80(5):926–930. doi: 10.1210/endo-80-5-926. [DOI] [PubMed] [Google Scholar]
  2. Bates P. C., Millward D. J. Characteristics of skeletal muscle growth and protein turnover in a fast-growing rat strain. Br J Nutr. 1981 Jul;46(1):7–13. doi: 10.1079/bjn19810004. [DOI] [PubMed] [Google Scholar]
  3. Bell G. E., Stern J. S. Evaluation of body composition of young obese and lean Zucker rats. Growth. 1977 Mar;41(1):63–80. [PubMed] [Google Scholar]
  4. Garlick P. J., McNurlan M. A., Preedy V. R. A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine. Biochem J. 1980 Nov 15;192(2):719–723. doi: 10.1042/bj1920719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Godbole V., York D. A., Bloxham D. P. Developmental changes in the fatty (fafa) rat: evidence for defective thermogenesis preceding the hyperlipogenesis and hyperinsulinaemia. Diabetologia. 1978 Jul;15(1):41–44. doi: 10.1007/BF01219327. [DOI] [PubMed] [Google Scholar]
  6. Martin R. J., Wangsness P. J., Gahagan J. H. Diurnal changes in serum metabolites and hormones in lean and obese Zucker rats. Horm Metab Res. 1978 May;10(3):187–192. doi: 10.1055/s-0028-1093431. [DOI] [PubMed] [Google Scholar]
  7. McGrath J. A., Goldspink D. F. The effects of cortisone treatment on the protein turnover of the soleus muscle after immobilization. Biochem Soc Trans. 1978;6(5):1017–1019. doi: 10.1042/bst0061017. [DOI] [PubMed] [Google Scholar]
  8. McNurlan M. A., Garlick P. J. Contribution of rat liver and gastrointestinal tract to whole-body protein synthesis in the rat. Biochem J. 1980 Jan 15;186(1):381–383. doi: 10.1042/bj1860381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Millward D. J., Garlick P. J., Nnanyelugo D. O., Waterlow J. C. The relative importance of muscle protein synthesis and breakdown in the regulation of muscle mass. Biochem J. 1976 Apr 15;156(1):185–188. doi: 10.1042/bj1560185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Odedra B. R., Dalal S. S., Millward D. J. Muscle protein synthesis in the streptozotocin-diabetic rat. A possible role for corticosterone in the insensitivity to insulin infusion in vivo. Biochem J. 1982 Feb 15;202(2):363–368. doi: 10.1042/bj2020363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Picou D., Reeds P. J., Jackson A., Poulter N. The measurement of muscle mass in children using [15N]creatine. Pediatr Res. 1976 Mar;10(3):184–188. doi: 10.1203/00006450-197603000-00008. [DOI] [PubMed] [Google Scholar]
  12. Pullar J. D., Webster A. J. Heat loss and energy retention during growth in congenitally obese and lean rats. Br J Nutr. 1974 May;31(3):377–392. doi: 10.1079/bjn19740046. [DOI] [PubMed] [Google Scholar]
  13. Pullar J. D., Webster A. J. The energy cost of fat and protein deposition in the rat. Br J Nutr. 1977 May;37(3):355–363. doi: 10.1079/bjn19770039. [DOI] [PubMed] [Google Scholar]
  14. Radcliffe J. D., Webster A. J. Sex, body composition and regulation of food intake during growth in the Zucker rat. Br J Nutr. 1978 May;39(3):483–492. doi: 10.1079/bjn19780064. [DOI] [PubMed] [Google Scholar]
  15. Rannels S. R., Jefferson L. S. Effects of glucocorticoids on muscle protein turnover in perfused rat hemicorpus. Am J Physiol. 1980 Jun;238(6):E564–E572. doi: 10.1152/ajpendo.1980.238.6.E564. [DOI] [PubMed] [Google Scholar]
  16. Santidrian S., Moreyra M., Munro H. N., Young V. R. Effect of corticosterone and its route of administration on muscle protein breakdown, measured in vivo by urinary excretion of N tau-methylhistidine in rats: response to different levels of dietary protein and energy. Metabolism. 1981 Aug;30(8):798–804. doi: 10.1016/0026-0495(81)90026-3. [DOI] [PubMed] [Google Scholar]
  17. Tomas F. M., Munro H. N., Young V. R. Effect of glucocorticoid administration on the rate of muscle protein breakdown in vivo in rats, as measured by urinary excretion of N tau-methylhistidine. Biochem J. 1979 Jan 15;178(1):139–146. doi: 10.1042/bj1780139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Waterlow J. C., Neale R. J., Rowe L., Palin I. Effects of diet and infection on creatine turnover in the rat. Am J Clin Nutr. 1972 Apr;25(4):371–375. doi: 10.1093/ajcn/25.4.371. [DOI] [PubMed] [Google Scholar]
  19. York D. A., Godbole V. Effect of adrenalectomy on obese 'fatty' rats. Horm Metab Res. 1979 Nov;11(11):646–646. doi: 10.1055/s-0028-1095808. [DOI] [PubMed] [Google Scholar]
  20. Yukimura Y., Bray G. A. Effects of adrenalectomy on body weight and the size and number of fat cells in the Zucker (fatty) rat. Endocr Res Commun. 1978;5(3):189–198. doi: 10.1080/07435807809083752. [DOI] [PubMed] [Google Scholar]

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