Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1982 Jun 15;204(3):647–652. doi: 10.1042/bj2040647

A macromolecular inhibitor of the antizyme to ornithine decarboxylase.

K Fujita, Y Murakami, S Hayashi
PMCID: PMC1158403  PMID: 7126159

Abstract

A macromolecular factor that inhibits the activity of the antizyme to ornithine decarboxylase (ODC) was found in rat liver extracts. The factor, 'antizyme inhibitor', was heat-labile, non diffusable and of similar molecular size to ODC. The antizyme inhibitor re-activated ODC that had been inactivated by antizyme, apparently by replacing ODC in a complex with antizyme. Therefore the antizyme inhibitor can be used to assay the amount of inactive ODC-antizyme complex formed in vitro. When assayed by this method, the complex was shown to be eluted before ODC from a Sephadex G-100 column. Significant increase in ODC activity was observed when the antizyme inhibitor was added to crude liver extracts from rats that had been injected with 1,3-diaminopropane to cause decay of ODC activity, suggesting the presence of inactive ODC-antizyme complex in the extracts.

Full text

PDF
647

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fong W. F., Heller J. S., Canellakis E. S. The appearance of an ornithine decarboxylase inhibitory protein upon the addition of putrescine to cell cultures. Biochim Biophys Acta. 1976 Apr 23;428(2):456–465. doi: 10.1016/0304-4165(76)90054-4. [DOI] [PubMed] [Google Scholar]
  2. Hannonen P., Raina A., Jänne J. Polyamine synthesis in the regenerating rat liver: stimulation of S-adenosyl methionine decarboxylase, and spermidine and spermine synthases after partial hepatectomy. Biochim Biophys Acta. 1972 Jun 26;273(1):84–90. doi: 10.1016/0304-4165(72)90194-8. [DOI] [PubMed] [Google Scholar]
  3. Hayashi S., Aramaki Y., Noguchi T. Diurnal change in ornithine decarboxylase activity of rat liver. Biochem Biophys Res Commun. 1972 Jan 31;46(2):795–800. doi: 10.1016/s0006-291x(72)80211-0. [DOI] [PubMed] [Google Scholar]
  4. Heller J. S., Canellakis E. S. Cellular control of ornithine decarboxylase activity by its antizyme. J Cell Physiol. 1981 May;107(2):209–217. doi: 10.1002/jcp.1041070206. [DOI] [PubMed] [Google Scholar]
  5. Heller J. S., Fong W. F., Canellakis E. S. Induction of a protein inhibitor to ornithine decarboxylase by the end products of its reaction. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1858–1862. doi: 10.1073/pnas.73.6.1858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Inoue H., Kato Y., Takigawa M., Adachi K., Takeda Y. Effect of DL-alpha-hydrazino-delta-aminovaleric acid, an inhibitor of ornithine decarboxylase, on polyamine metabolism in isoproterenol-stimulated mouse parotid glands. J Biochem. 1975 Apr;77(4):879–893. doi: 10.1093/oxfordjournals.jbchem.a130796. [DOI] [PubMed] [Google Scholar]
  7. Jänne J., Pösö H., Raina A. Polyamines in rapid growth and cancer. Biochim Biophys Acta. 1978 Apr 6;473(3-4):241–293. doi: 10.1016/0304-419x(78)90015-x. [DOI] [PubMed] [Google Scholar]
  8. Kallio A., Löfman M., Pösö H., Jänne J. Diamine-induced inhibition of liver ornithine decarboxylase. Biochem J. 1979 Jan 1;177(1):63–69. doi: 10.1042/bj1770063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kameji T., Murakami Y., Fujita K., Noguchi T., Hayashi S. Rat liver ornithine decarboxylase purification and some immunochemical studies. Med Biol. 1981 Dec;59(5-6):296–299. [PubMed] [Google Scholar]
  10. Kyriakidis D. A., Heller J. S., Canellakis E. S. Modulation of ornithine decarboxylase activity in Escherichia coli by positive and negative effectors. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4699–4703. doi: 10.1073/pnas.75.10.4699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maudsley D. V. Regulation of polyamine biosynthesis. Biochem Pharmacol. 1979;28(2):153–161. doi: 10.1016/0006-2952(79)90496-9. [DOI] [PubMed] [Google Scholar]
  12. McCann P. P., Tardif C., Hornsperger J. M., Böhlen P. Two distinct mechanisms for ornithine decarboxylase regulation by polyamines in rat hepatoma cells. J Cell Physiol. 1979 May;99(2):183–190. doi: 10.1002/jcp.1040990204. [DOI] [PubMed] [Google Scholar]
  13. Miller J. V., Jr, Cuatrecasas P., Thompson E. B. Purification of tyrosine aminotransferase by affinity chromatography. Biochim Biophys Acta. 1972 Aug 28;276(2):407–415. doi: 10.1016/0005-2744(72)91001-7. [DOI] [PubMed] [Google Scholar]
  14. Morris D. R., Fillingame R. H. Regulation of amino acid decarboxylation. Annu Rev Biochem. 1974;43(0):303–325. doi: 10.1146/annurev.bi.43.070174.001511. [DOI] [PubMed] [Google Scholar]
  15. Pösö H., Kallio A., Scalabrino G., Jänne J. Specific inhibition of the synthesis of putrescine and spermidine by 1,3-diaminopropane in rat liver in vivo. Biochim Biophys Acta. 1977 Mar 29;497(1):288–297. doi: 10.1016/0304-4165(77)90162-3. [DOI] [PubMed] [Google Scholar]
  16. Russell D. H., Snyder S. H. Amine synthesis in regenerating rat liver: extremely rapid turnover of ornithine decarboxylase. Mol Pharmacol. 1969 May;5(3):253–262. [PubMed] [Google Scholar]
  17. Russell D. H., Snyder S. H., Medina V. J. Growth hormone induction of ornithine decarboxylase in rat liver. Endocrinology. 1970 Jun;86(6):1414–1419. doi: 10.1210/endo-86-6-1414. [DOI] [PubMed] [Google Scholar]
  18. Sawayama T., Kinugasa H., Nishimura H. Syntheses of ornithine decarboxylase inhibitors: D- and DL-alpha-hydrazinoornithine. Chem Pharm Bull (Tokyo) 1976 Feb;24(2):326–329. doi: 10.1248/cpb.24.326. [DOI] [PubMed] [Google Scholar]
  19. Schrock T. R., Oakman N. J., Bucher N. L. Ornithine decarboxylase activity in relation to growth of rat liver. Effects of partial hepatectomy, hypertonic infusions, celite injection or other stressful procedures. Biochim Biophys Acta. 1970 Apr 15;204(2):564–577. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES