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. 1982 Jul 15;206(1):161–164. doi: 10.1042/bj2060161

Association of epididymal secretory proteins showing alpha-lactalbumin-like activity with the plasma membrane of rat spermatozoa.

R Jones, C R Brown
PMCID: PMC1158563  PMID: 7126189

Abstract

Three hormonally regulated proteins with mol.wts of 18 500, 19 000 and 23 000 have been shown to associate with the plasma membrane of spermatozoa during maturation in the epididymis. All three proteins showed some alpha-lactalbumin-like activity, suggesting that they may act in concert with epididymal glycosidases and glycosyltransferases to regulate the modification of sugars on membrane-bound glycoproteins.

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Selected References

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  1. D'Agostino A., Jones R., White R., Parker M. G. Androgenic regulation of messenger RNA in rat epididymis. Biochem J. 1980 Sep 15;190(3):505–512. doi: 10.1042/bj1900505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fitzgerald D. K., Colvin B., Mawal R., Ebner K. E. Enzymic assay for galactosyl transferase activity of lactose synthetase and alpha-lactalbumin in purified and crude systems. Anal Biochem. 1970 Jul;36(1):43–61. doi: 10.1016/0003-2697(70)90330-1. [DOI] [PubMed] [Google Scholar]
  3. Fox N., Damjanov I., Knowles B. B., Solter D. Teratocarcinoma antigen is secreted by epididymal cells and coupled to maturing sperm. Exp Cell Res. 1982 Feb;137(2):485–488. doi: 10.1016/0014-4827(82)90058-1. [DOI] [PubMed] [Google Scholar]
  4. Gaunt S. J. A 28K-dalton cell surface autoantigen of spermatogenesis: characterization using a monoclonal antibody. Dev Biol. 1982 Jan;89(1):92–100. doi: 10.1016/0012-1606(82)90297-4. [DOI] [PubMed] [Google Scholar]
  5. Hamilton D. W. Evidence for alpha-lactalbumin-like activity in reproductive tract fluids of the male rat. Biol Reprod. 1981 Sep;25(2):385–392. doi: 10.1095/biolreprod25.2.385. [DOI] [PubMed] [Google Scholar]
  6. Hamilton D. W. UDP-galactose:N-acetylglucosamine galactosyltransferase in fluids from rat rete testis and epididymis. Biol Reprod. 1980 Sep;23(2):377–385. doi: 10.1095/biolreprod23.2.377. [DOI] [PubMed] [Google Scholar]
  7. Jones J. B., Jolly J. D., Dunn C. D., Lange R. D. The in vitro growth of erythroid colonies from dog bone marrow. Can J Comp Med. 1979 Oct;43(4):365–370. [PMC free article] [PubMed] [Google Scholar]
  8. Jones R., Brown C. R., Von Glós K. I., Parker M. G. Hormonal regulation of protein synthesis in the rat epididymis. Characterization of androgen-dependent and testicular fluid-dependent proteins. Biochem J. 1980 Jun 15;188(3):667–676. doi: 10.1042/bj1880667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jones R., Pholpramool C., Setchell B. P., Brown C. R. Labelling of membrane glycoproteins on rat spermatozoa collected from different regions of the epididymis. Biochem J. 1981 Nov 15;200(2):457–460. doi: 10.1042/bj2000457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones R., von Glos K. I., Brown C. R. Characterization of hormonally regulated secretory proteins from the caput epididymidis of the rabbit. Biochem J. 1981 Apr 15;196(1):105–114. doi: 10.1042/bj1960105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lopo A. C., Vacquier V. D. Antibody to a sperm surface glycoprotein inhibits the egg jelly-induced acrosome reaction of sea urchin sperm. Dev Biol. 1980 Oct;79(2):325–333. doi: 10.1016/0012-1606(80)90119-0. [DOI] [PubMed] [Google Scholar]
  12. Owen M. J., Knott J. C., Crumpton M. J. Labeling of lymphocyte surface antigens by the lipophilic, photoactivatable reagent hexanoyldiiodo-N-(4-azido-2-nitrophenyl)tyramine. Biochemistry. 1980 Jun 24;19(13):3092–3099. doi: 10.1021/bi00554a040. [DOI] [PubMed] [Google Scholar]
  13. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

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