Fig. 1.

Effects of dietary supplementation with Hermetia illucens meal (HIM) on hepatic FXR signaling pathway (WP2879). a) The pathway created was extensively modified from the original pathway to indicate significant (p < 0.05) differentially regulated genes. Coloured squares with the gene name represent the gene expression ratio in the HIM3 fed group with respect to the control diet (HIM0). Up-regulation of genes is indicated by a red coloured box; blue box indicates down-regulation; gray indicates genes that were not investigated. The darker the color, the greater the statistical significance. b) Putative FXRE sequences identified in the PPARα promoters of rainbow trout by using the NUBIScan tool (Version 2.0). c) Fold change in target gene expression in HIM3 fed fish normalized to the internal control gene (18s rRNA) and relative to the control diet (HIM0). Results of the real-time PCR data were represented as mean values ± 95 % confidence intervals.