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. 1982 Sep 15;206(3):433–439. doi: 10.1042/bj2060433

The effects in vitro of hypoglycaemia and recovery from anoxia on synaptosomal metabolism.

S A Harvey, R F Booth, J B Clark
PMCID: PMC1158608  PMID: 6816223

Abstract

Synaptosomes from several regions of the rat brain were found to exhibit half-maximal rates of 14CO2 output and [14C]acetylcholine synthesis from D-[U-14C]glucose at glucose concentrations approx. 50-fold lower than those required by the brain in situ. However, synaptosomal acetylcholine synthesis was found not to be directly proportional to substrate oxidation as measured by 14CO2 output. When synaptosomes had been exposed to anoxia in vitro, their metabolic indices (14CO2 and [14C]acetylcholine synthesis, and adenine nucleotide levels) were found not to be significantly different from control aerobic values, unless they had been subjected to veratridine depolarization. This is in accord with previous findings that neither the absolute metabolic rates nor the vulnerability to hypoxic damage exhibited by brain in situ is reflected by brain slices in vitro, unless these are stimulated by depolarization. The use of synaptosomes as a model for synaptic damage in vivo is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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