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. 1982 Sep 15;206(3):605–609. doi: 10.1042/bj2060605

Superoxide-dependent formation of hydroxyl radicals and lipid peroxidation in the presence of iron salts. Detection of `catalytic' iron and anti-oxidant activity in extracellular fluids

John M C Gutteridge *, David A Rowley †, Barry Halliwell †
PMCID: PMC1158629  PMID: 6293469

Abstract

Synovial fluid from rheumatoid patients and normal cerebrospinal fluid contains micromolar concentrations of non-protein-bound iron salts that can promote lipid peroxidation and also the superoxide-dependent formation of hydroxyl radicals from hydrogen peroxide. These iron catalysts of oxygen radical reactions cannot be detected by conventional assays unless interfering high-molecular-weight substances, probably proteins, are removed by ultrafiltration or inactivated by exposure to low pH values. The bleomycin assay for `catalytic' iron [Gutteridge, Rowley & Halliwell (1981) Biochem. J. 199, 263–265] does not suffer from these artifacts.

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Selected References

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