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. 1982 Sep 15;206(3):619–626. doi: 10.1042/bj2060619

The effect of alpha-adrenergic agonists on the membrane potential of fat-cell mitochondria in situ.

R J Davis, B R Martin
PMCID: PMC1158631  PMID: 6128974

Abstract

1. The accumulation of [3H]methyltriphenylphosphonium by isolated fat-cells was used to estimate the membrane potential of mitochondria in situ. 2. An alpha-adrenergic receptor-mediated decrease in the apparent accumulation of [3H]methyltriphenylphosphonium was observed. Methoxamine, clonidine and low concentrations of phenylephrine decreased the calculated mitochrondrial membrane potential without significantly raising cyclic AMP levels, adenylate cyclase activity or stimulating lipolysis. The agonist potency order was phenylephrine greater than methoxamine greater than clonidine. 3. The decrease in the calculated mitochondrial membrane potential caused by phenylephrine, clonidine and methoxamine was blocked by the alpha-adrenergic antagonist prazosin but not by yohimbine nor by the beta-antagonist propranolol. This suggests that the effect on the calculated mitochondrial membrane potential may be mediated by alpha 1-like receptors.

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Selected References

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  1. Althaus-Salzmann M., Carafoli E., Jakob A. Ca2+, K+ redistributions and alpha-adrenergic activation of glycogenolysis in perfused rat livers. Eur J Biochem. 1980 May;106(1):241–248. doi: 10.1111/j.1432-1033.1980.tb06015.x. [DOI] [PubMed] [Google Scholar]
  2. Belsham G. J., Denton R. M., Tanner M. J. Use of a novel rapid preparation of fat-cell plasma membranes employing Percoll to investigate the effects of insulin and adrenaline on membrane protein phosphorylation within intact fat-cells. Biochem J. 1980 Nov 15;192(2):457–467. doi: 10.1042/bj1920457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berthelsen S., Pettinger W. A. A functional basis for classification of alpha-adrenergic receptors. Life Sci. 1977 Sep 1;21(5):595–606. doi: 10.1016/0024-3205(77)90066-2. [DOI] [PubMed] [Google Scholar]
  4. Brown B. L., Ekins R. P., Albano J. D. Saturation assay for cyclic AMP using endogenous binding protein. Adv Cyclic Nucleotide Res. 1972;2:25–40. [PubMed] [Google Scholar]
  5. Burgess G. M., Claret M., Jenkinson D. H. Effects of catecholamines, ATP and ionophore A23187 on potassium and calcium movements in isolated hepatocytes. Nature. 1979 Jun 7;279(5713):544–546. doi: 10.1038/279544a0. [DOI] [PubMed] [Google Scholar]
  6. Davis R. J., Brand M. D., Martin B. R. The effect of insulin on plasma-membrane and mitochondrial-membrane potentials in isolated fat-cells. Biochem J. 1981 Apr 15;196(1):133–147. doi: 10.1042/bj1960133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davis R. J., Martin B. R. The effect of beta-adrenergic agonists on the membrane potential of fat-cell mitochondria in situ. Biochem J. 1982 Sep 15;206(3):611–618. doi: 10.1042/bj2060611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Deutsch C., Erecińska M., Werrlein R., Silver I. A. Cellular energy metabolism, trans-plasma and trans-mitochondrial membrane potentials, and pH gradients in mouse neuroblastoma. Proc Natl Acad Sci U S A. 1979 May;76(5):2175–2179. doi: 10.1073/pnas.76.5.2175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Drew G. M. Pharmacological characterisation of the presynaptic alpha-adrenoceptor in the rat vas deferens. Eur J Pharmacol. 1977 Mar 21;42(2):123–130. doi: 10.1016/0014-2999(77)90351-x. [DOI] [PubMed] [Google Scholar]
  10. Drew G. M. Pharmacological characterization of the presynaptic alpha-adrenoceptors regulating cholinergic activity in the guinea-pig ileum. Br J Pharmacol. 1978 Oct;64(2):293–300. doi: 10.1111/j.1476-5381.1978.tb17303.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Exton J. H. Molecular mechanisms involved in alpha-adrenergic responses. Mol Cell Endocrinol. 1981 Sep;23(3):233–264. doi: 10.1016/0303-7207(81)90123-4. [DOI] [PubMed] [Google Scholar]
  12. Fink S. A., Williams J. A. Adrenergic receptors mediating depolarization in brown adipose tissue. Am J Physiol. 1976 Sep;231(3):700–706. doi: 10.1152/ajplegacy.1976.231.3.700. [DOI] [PubMed] [Google Scholar]
  13. GARLAND P. B., RANDLE P. J. A rapid enzymatic assay for glycerol. Nature. 1962 Dec 8;196:987–988. doi: 10.1038/196987a0. [DOI] [PubMed] [Google Scholar]
  14. García-Sáinz J. A., Fain J. N. Effect of insulin, catecholamines and calcium ions on phospholipid metabolism in isolated white fat-cells. Biochem J. 1980 Mar 15;186(3):781–789. doi: 10.1042/bj1860781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Giudicelli Y., Thotakura N. R., Lacasa D., Pecquery R., Agli B. Direct binding studies do not support the existence of true alpha-adreno-receptors in rat white fat cells. Biochem Biophys Res Commun. 1981 May 29;100(2):621–628. doi: 10.1016/s0006-291x(81)80221-5. [DOI] [PubMed] [Google Scholar]
  16. Haylett D. G., Jenkinson D. H. Effects of noradrenaline on potassium reflux, membrane potential and electrolyte levels in tissue slices prepared from guinea-pig liver. J Physiol. 1972 Sep;225(3):721–750. doi: 10.1113/jphysiol.1972.sp009966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hoek J. B., Nicholls D. G., Williamson J. R. Determination of the mitochondrial protonmotive force in isolated hepatocytes. J Biol Chem. 1980 Feb 25;255(4):1458–1464. [PubMed] [Google Scholar]
  18. Hoffman B. B., De Lean A., Wood C. L., Schocken D. D., Lefkowitz R. J. Alpha-adrenergic receptor subtypes: quantitative assessment by ligand binding. Life Sci. 1979 May 7;24(19):1739–1745. doi: 10.1016/0024-3205(79)90061-4. [DOI] [PubMed] [Google Scholar]
  19. Hoffman B. B., Mullikin-Kilpatrick D., Lefkowitz R. J. Heterogeneity of radioligand binding to alpha-adrenergic receptors. Analysis of guanine nucleotide regulation of agonist binding in relation to receptor subtypes. J Biol Chem. 1980 May 25;255(10):4645–4652. [PubMed] [Google Scholar]
  20. Kessar P., Crompton M. The alpha-adrenergic-mediated activation of Ca2+ influx into cardiac mitochondria. A possible mechanism for the regulation of intramitochondrial free CA2+. Biochem J. 1981 Nov 15;200(2):379–388. doi: 10.1042/bj2000379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Langer S. Z. Presynaptic regulation of catecholamine release. Biochem Pharmacol. 1974 Jul 1;23(13):1793–1800. doi: 10.1016/0006-2952(74)90187-7. [DOI] [PubMed] [Google Scholar]
  22. Lawrence J. C., Jr, Larner J. Effects of insulin, methoxamine, and calcium on glycogen synthase in rat adipocytes. Mol Pharmacol. 1978 Nov;14(6):1079–1091. [PubMed] [Google Scholar]
  23. Lawrence J. C., Jr, Larner J. Evidence for alpha adrenergic activation of phosphorylase and inactivation of glycogen synthase in rat adipocytes. Effects of alpha and beta adrenergic agonists and antagonists on glycogen synthase and phosphorylase. Mol Pharmacol. 1977 Nov;13(6):1060–1075. [PubMed] [Google Scholar]
  24. Luzio J. P., Jones R. C., Siddle K., Hales C. N. Dissociation of the effect of adrenalin on glucose uptake from that on adenosine cyclic 3',5'-monophosphate levels and on lipolysis in rat-isolated fat cells. Biochim Biophys Acta. 1974 Aug 7;362(1):29–36. doi: 10.1016/0304-4165(74)90022-1. [DOI] [PubMed] [Google Scholar]
  25. Martin B. R., Voorheis H. P. A simple enzymic method for the synthesis of adenosine 5'-[alpha-32P]triphosphate on a preparative scale. Biochem J. 1977 Mar 1;161(3):555–559. doi: 10.1042/bj1610555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Mitchell P., Moyle J. Estimation of membrane potential and pH difference across the cristae membrane of rat liver mitochondria. Eur J Biochem. 1969 Feb;7(4):471–484. doi: 10.1111/j.1432-1033.1969.tb19633.x. [DOI] [PubMed] [Google Scholar]
  27. Murphy E., Coll K., Rich T. L., Williamson J. R. Hormonal effects on calcium homeostasis in isolated hepatocytes. J Biol Chem. 1980 Jul 25;255(14):6600–6608. [PubMed] [Google Scholar]
  28. Nicholls D. G., Crompton M. Mitochondrial calcium transport. FEBS Lett. 1980 Mar 10;111(2):261–268. doi: 10.1016/0014-5793(80)80806-4. [DOI] [PubMed] [Google Scholar]
  29. Pecquery R., Giudicelli Y. Heterogeneity and subcellular localization of hamster adipocyte alpha-adrenergic receptors: evidence of alpha 1- and alpha 2-subtypes. FEBS Lett. 1980 Jul 11;116(1):85–90. doi: 10.1016/0014-5793(80)80534-5. [DOI] [PubMed] [Google Scholar]
  30. Perry M. C., Hales C. N. Factors affecting the permeability of isolated fat-cells from the rat to [42K] potassium and [36Cl] chloride ions. Biochem J. 1970 Apr;117(3):615–621. doi: 10.1042/bj1170615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Poggioli J., Berthon B., Claret M. Calcium movements in in situ mitochondria following activation of alpha-adrenergic receptors in rat liver cells. FEBS Lett. 1980 Jun 30;115(2):243–246. doi: 10.1016/0014-5793(80)81178-1. [DOI] [PubMed] [Google Scholar]
  32. Rodbell M., Lin M. C., Salomon Y., Londos C., Harwood J. P., Martin B. R., Rendell M., Berman M. Role of adenine and guanine nucleotides in the activity and response of adenylate cyclase systems to hormones: evidence for multisite transition states. Adv Cyclic Nucleotide Res. 1975;5:3–29. [PubMed] [Google Scholar]
  33. Salomon Y., Londos C., Rodbell M. A highly sensitive adenylate cyclase assay. Anal Biochem. 1974 Apr;58(2):541–548. doi: 10.1016/0003-2697(74)90222-x. [DOI] [PubMed] [Google Scholar]
  34. Scott I. D., Nicholls D. G. Energy transduction in intact synaptosomes. Influence of plasma-membrane depolarization on the respiration and membrane potential of internal mitochondria determined in situ. Biochem J. 1980 Jan 15;186(1):21–33. doi: 10.1042/bj1860021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Starke K., Endo T., Taube H. D. Relative pre- and postsynaptic potencies of alpha-adrenoceptor agonists in the rabbit pulmonary artery. Naunyn Schmiedebergs Arch Pharmacol. 1975;291(1):55–78. doi: 10.1007/BF00510821. [DOI] [PubMed] [Google Scholar]
  36. Stein J. M., Hales C. N. Effect of adrenaline on 32 P incorporation into rat fat-cell phospholipids. Biochem J. 1972 Jul;128(3):531–541. doi: 10.1042/bj1280531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Stein J. M. The effect of adrenaline and of alpha- and beta-adrenergic blocking agents on ATP concentration and on incorporation of 32Pi into ATP in rat fat cells. Biochem Pharmacol. 1975 Sep 15;24(18):1659–1662. doi: 10.1016/0006-2952(75)90002-7. [DOI] [PubMed] [Google Scholar]
  38. Taylor W. M., Prpić V., Exton J. H., Bygrave F. L. Stable changes to calcium fluxes in mitochondria isolated from rat livers perfused with alpha-adrenergic agonists and with glucagon. Biochem J. 1980 May 15;188(2):443–450. doi: 10.1042/bj1880443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. U'Prichard D. C., Charness M. E., Robertson D., Snyder S. H. Prazosin: differential affinities for two populations of alpha-noradrenergic receptor binding sites. Eur J Pharmacol. 1978 Jul 1;50(1):87–89. doi: 10.1016/0014-2999(78)90258-3. [DOI] [PubMed] [Google Scholar]
  40. Wood C. L., Arnett C. D., Clarke W. R., Tsai B. S., Lefkowitz R. J. Subclassification of alpha-adrenergic receptors by direct binding studies. Biochem Pharmacol. 1979 Apr 15;28(8):1277–1282. doi: 10.1016/0006-2952(79)90424-6. [DOI] [PubMed] [Google Scholar]

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