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. 1996 Feb 1;490(Pt 3):577–583. doi: 10.1113/jphysiol.1996.sp021168

Two different constituents of plasma increase cytosolic calcium selectively in neurons or glia of primary rat cerebellar cultures.

L Nuñez 1, J García-Sancho 1
PMCID: PMC1158697  PMID: 8683458

Abstract

1. The ability of several serum fractions to increase the cytosolic calcium concentration ([Ca2+]i) was tested in rat cerebellar cells maintained in primary culture. 2. Serum filtered through an ultrafiltration membrane with 3000 Da molecular mass cut-off (filtered serum, FS) selectively stimulated neurons whereas dialysed serum (DS) selectively stimulated glia. 3. The effects of FS were due to glutamate as they were reproduced by N-methyl-D-aspartate (NMDA), blocked by NMDA receptor antagonists and prevented by enzymatic removal of glutamate. 4. The effects of DS on glia were not reproduced by platelet-activating factor, thrombin or bradykinin. They were not lost on heating or extraction with diethyl ether. They were reproduced by a methanol-chloroform-HCl extract from DS and by several commercial fraction V plasma albumins. 5. These [Ca2+]i-increasing factors present in blood could contribute to brain damage during ischaemia if they reached the brain interstitium on disruption of the blood-brain barrier.

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Selected References

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