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. 1996 Mar 1;491(Pt 2):465–469. doi: 10.1113/jphysiol.1996.sp021230

Effects of immunization against an autocrine inhibitor of milk secretion in lactating goats.

C J Wilde 1, C V Addey 1, M Peaker 1
PMCID: PMC1158740  PMID: 8866869

Abstract

1. Lactating goats were immunized against the goat's milk protein identified as a feedback inhibitor of lactation (FIL). Immunization was by three treatments during the declining stage of lactation. 2. When antibodies to FIL were consistently detected in milk (in response to the third treatment), the rate of decline in milk secretion was significantly reduced compared with sham-immunized controls. Such a response was not apparent with the first two treatments when serum but not milk titres of anti-FIL were raised. 3. When one gland of immunized goats was switched (after the third immunization) from twice- to once-daily milking, the ipsilateral decrease in the rate of milk secretion was reduced significantly compared with sham-immunized goats. 4. The results are compatible with the hypothesis that the autocrine agent FIL acts during milk accumulation as an inhibitor of milk secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Byars N. E. An adjuvant formulation that selectively elicits the formation of antibodies of protective isotypes and of cell-mediated immunity. J Immunol Methods. 1986 Dec 24;95(2):157–168. doi: 10.1016/0022-1759(86)90402-3. [DOI] [PubMed] [Google Scholar]
  2. Blatchford D. R., Peaker M. Effects of frequent milking on milk secretion during lactation in the goat: relation to factors which limit the rate of secretion. Q J Exp Physiol. 1982 Apr;67(2):303–310. doi: 10.1113/expphysiol.1982.sp002638. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Henderson A. J., Blatchford D. R., Peaker M. The effects of milking thrice instead of twice daily on milk secretion in the goat. Q J Exp Physiol. 1983 Oct;68(4):645–652. doi: 10.1113/expphysiol.1983.sp002754. [DOI] [PubMed] [Google Scholar]
  5. Henderson A. J., Peaker M. Feed-back control of milk secretion in the goat by a chemical in milk. J Physiol. 1984 Jun;351:39–45. doi: 10.1113/jphysiol.1984.sp015230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pankey J. W., Boddie N. T., Watts J. L., Nickerson S. C. Evaluation of protein A and a commercial bacterin as vaccines against Staphylococcus aureus mastitis by experimental challenge. J Dairy Sci. 1985 Mar;68(3):726–731. doi: 10.3168/jds.S0022-0302(85)80879-1. [DOI] [PubMed] [Google Scholar]
  7. Peaker M. The effect of raised intramammary pressure on mammary function in the goat in relation to the cessation of lactation. J Physiol. 1980 Apr;301:415–428. doi: 10.1113/jphysiol.1980.sp013214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rennison M. E., Kerr M., Addey C. V., Handel S. E., Turner M. D., Wilde C. J., Burgoyne R. D. Inhibition of constitutive protein secretion from lactating mouse mammary epithelial cells by FIL (feedback inhibitor of lactation), a secreted milk protein. J Cell Sci. 1993 Oct;106(Pt 2):641–648. doi: 10.1242/jcs.106.2.641. [DOI] [PubMed] [Google Scholar]
  9. Sheldrake R. F., Husband A. J., Watson D. L., Cripps A. W. The effect of intraperitoneal and intramammary immunization of sheep on the numbers of antibody-containing cells in the mammary gland, and antibody titres in blood serum and mammary secretions. Immunology. 1985 Dec;56(4):605–614. [PMC free article] [PubMed] [Google Scholar]
  10. Wilde C. J., Addey C. V., Boddy L. M., Peaker M. Autocrine regulation of milk secretion by a protein in milk. Biochem J. 1995 Jan 1;305(Pt 1):51–58. doi: 10.1042/bj3050051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wilde C. J., Calvert D. T., Daly A., Peaker M. The effect of goat milk fractions on synthesis of milk constituents by rabbit mammary explants and on milk yield in vivo. Evidence for autocrine control of milk secretion. Biochem J. 1987 Feb 15;242(1):285–288. doi: 10.1042/bj2420285. [DOI] [PMC free article] [PubMed] [Google Scholar]

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