Table 1.

Primer sequence, product size, and annealing temperature for quantitative RT-PCR.

Target Gene (Abbreviation)	Forward (Top) and Reverse (Bottom) Primer Sequences	Size of AMPLIFIED Products (bp)	Annealing Temperature (°C)	Sequence ID
b-actin	5′-ATTGCCGACAGGATGCAGA-3′	89	60	NM_001101.3
	5′-GAGTACTTGCGCTCAGGAGGA-3′
ALP	5′-CTCTATCTTTGGTCTGGCCC-3′	154	60	NM_001177520.3
	5′-CTGCGCCTGGTAGTTGTTGT-3′
BMP2	5′-TCCACTAATCATGCCATTGTTCA-3′	73	60	NM_001200.4
	5′-GGGACACAGCATGCCTTAGGA-3′
OCN	5′-GTGCAGAGTCCAGCAAAGGT-3′	175	60	NM_199173.6
	5′-TCAGCCAACTCGTCACAGTC-3′
OPN	5′-ACACATATGATGGCCGAGGTGA-3′	115	60	NM_000582.3
	5′-TGTGAGGTGATGTCCTCGTCTGT-3′
RUNX2	5′-AACCCTTAATTTGCACTGGGTCA-3′	145	60	NM_001024630.3
	5′-CAAATTCCAGCAATGTTTGTGCTAC-3′