Figure 2.

Effect of PDREP on the expression of MMPs and inflammatory cytokine-related genes, and NO and PGE2 production in SW1353 cells. (A) SW1353 cells (3 × 10⁵ cells/well) were seeded in 6-well plates, treated with PDREP (0, 30, 100, or 300 µg/mL) or MSM (300 µg/mL), and then stimulated with IL-1β (0.5 ng/mL) for 24 h. The expression of genes (MMP1, MMP3, MMP13, Col2a1, TNF-α, and IL6) was evaluated by qRT-PCR. (B) SW1353 cells (2 × 10⁴ cells/well) were dispensed into a 48-well plate. The cells were then treated with PDREP (0, 30, 100, or 300 µg/mL) or MSM (300 µg/mL), followed by treatment with IL-1β (0.5 ng/mL), and cultured for 24 h. The levels of NO and PGE2 in the culture supernatant after incubation were quantified using ELISA kits following the manufacturer’s protocol. The experiments were conducted in triplicate, and the error bars represent the standard deviation. Significant differences between the PDREP and control groups are indicated (p < 0.05). Bars labeled with different superscript letters indicate p values < 0.05.