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. 1997 Sep 1;503(Pt 2):435–443. doi: 10.1111/j.1469-7793.1997.435bh.x

Spatial variation in sympathetic influences on the vasculature of the synovium and medial collateral ligament of the rabbit knee joint.

J J McDougall 1, W R Ferrell 1, R C Bray 1
PMCID: PMC1159874  PMID: 9306284

Abstract

1. Laser Doppler perfusion imaging was used to assess the role of the sympathetic nervous system in the control of blood flow to the medial collateral ligament and capsule (synovium and overlying fibrous tissues) of the rabbit knee joint. 2. Electrical stimulation of the saphenous nerve (width 1 ms; amplitude 20V; 1-30 Hz) produced a frequency-dependent vasoconstriction of knee joint vasculature. The response was maximal at 30 Hz and gave the greatest fall in perfusion at the femoral insertion of the ligament (by 33.8 +/- 7.4%, mean +/- S.E.M.; n = 5-6) and the smallest decrease at the tibial insertion of the ligament (by 10.6 +/- 2.9%). 3. Topical application of phentolamine (10(-6) mol) had no significant effect on basal knee joint blood flow. However, it abolished the nerve-mediated constrictor responses in all regions of the medial collateral ligament and synovium at all frequencies. 4. Topical administration of adrenaline (10(-14) to 10(-7) mol) caused a dose-dependent decrease in knee joint blood flow with the highest dose producing > 75% reduction in perfusion at all areas. 5. There was no evidence of a reactive hyperaemia in the 5 min following a 5 min period of femoral artery occlusion. Artificial manipulation of arterial blood pressure by intravenous infusion or withdrawal of blood caused a proportional change in ligament and synovial blood flow. These observations may indicate a lack of autoregulation in the joint and its exclusion from baroreflex modulation. 6. These results suggest a potential role for the sympathetic nervous system in the control of knee joint blood flow. Neuromodulation of ligament perfusion appears to predominate at the femoral insertion and this could prove to have functional significance.

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Selected References

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  1. Anderson R. R., Parrish J. A. The optics of human skin. J Invest Dermatol. 1981 Jul;77(1):13–19. doi: 10.1111/1523-1747.ep12479191. [DOI] [PubMed] [Google Scholar]
  2. Bray R. C., Butterwick D. J., Doschak M. R., Tyberg J. V. Coloured microsphere assessment of blood flow to knee ligaments in adult rabbits: effects of injury. J Orthop Res. 1996 Jul;14(4):618–625. doi: 10.1002/jor.1100140417. [DOI] [PubMed] [Google Scholar]
  3. Bray R. C., Fisher A. W., Frank C. B. Fine vascular anatomy of adult rabbit knee ligaments. J Anat. 1990 Oct;172:69–79. [PMC free article] [PubMed] [Google Scholar]
  4. Bray R., Forrester K., McDougall J. J., Damji A., Ferrell W. R. Evaluation of laser Doppler imaging to measure blood flow in knee ligaments of adult rabbits. Med Biol Eng Comput. 1996 May;34(3):227–231. doi: 10.1007/BF02520078. [DOI] [PubMed] [Google Scholar]
  5. COBBOLD A. F., LEWIS O. J. The action of adrenaline, noradrenaline and acetylcholine on blood flow through joints. J Physiol. 1956 Aug 28;133(2):472–474. doi: 10.1113/jphysiol.1956.sp005602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. COBBOLD A. F., LEWIS O. J. The nervous control of joint blood vessels. J Physiol. 1956 Aug 28;133(2):467–471. doi: 10.1113/jphysiol.1956.sp005601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chimich D., Shrive N., Frank C., Marchuk L., Bray R. Water content alters viscoelastic behaviour of the normal adolescent rabbit medial collateral ligament. J Biomech. 1992 Aug;25(8):831–837. doi: 10.1016/0021-9290(92)90223-n. [DOI] [PubMed] [Google Scholar]
  8. Chowdhury P., Matyas J. R., Frank C. B. The "epiligament" of the rabbit medial collateral ligament: a quantitative morphological study. Connect Tissue Res. 1991;27(1):33–50. doi: 10.3109/03008209109006993. [DOI] [PubMed] [Google Scholar]
  9. Dick W. C., Jubb R., Buchanan W. W., Williamson J., Whaley K., Porter B. B. Studies on the sympathetic control of normal and diseased synovial blood vessels: the effect of alpha and beta receptor stimulation and inhibition, monitored by the 133xenon clearance technique. Clin Sci. 1971 Feb;40(2):197–209. doi: 10.1042/cs0400197. [DOI] [PubMed] [Google Scholar]
  10. Ferrell W. R., Najafipour H. Changes in synovial PO2 and blood flow in the rabbit knee joint due to stimulation of the posterior articular nerve. J Physiol. 1992 Apr;449:607–617. doi: 10.1113/jphysiol.1992.sp019104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Frank C., McDonald D., Lieber R., Sabiston P. Biochemical heterogeneity within the maturing rabbit medial collateral ligament. Clin Orthop Relat Res. 1988 Nov;(236):279–285. [PubMed] [Google Scholar]
  12. Gollehon D. L., Torzilli P. A., Warren R. F. The role of the posterolateral and cruciate ligaments in the stability of the human knee. A biomechanical study. J Bone Joint Surg Am. 1987 Feb;69(2):233–242. [PubMed] [Google Scholar]
  13. Jacobsen K. Osteoarthrosis following insufficiency of the cruciate ligaments in man. A clinical study. Acta Orthop Scand. 1977;48(5):520–526. doi: 10.3109/17453677708989742. [DOI] [PubMed] [Google Scholar]
  14. Karimian S. M., McDougall J. J., Ferrell W. R. Neuropeptidergic and autonomic control of the vasculature of the rat knee joint revealed by laser Doppler perfusion imaging. Exp Physiol. 1995 May;80(3):341–348. doi: 10.1113/expphysiol.1995.sp003851. [DOI] [PubMed] [Google Scholar]
  15. Khoshbaten A., Ferrell W. R. Alterations in cat knee joint blood flow induced by electrical stimulation of articular afferents and efferents. J Physiol. 1990 Nov;430:77–86. doi: 10.1113/jphysiol.1990.sp018282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Knight A. D., Levick J. R. The density and distribution of capillaries around a synovial cavity. Q J Exp Physiol. 1983 Oct;68(4):629–644. doi: 10.1113/expphysiol.1983.sp002753. [DOI] [PubMed] [Google Scholar]
  17. Lam F. Y., Ferrell W. R. Acute inflammation in the rat knee joint attenuates sympathetic vasoconstriction but enhances neuropeptide-mediated vasodilatation assessed by laser Doppler perfusion imaging. Neuroscience. 1993 Jan;52(2):443–449. doi: 10.1016/0306-4522(93)90170-k. [DOI] [PubMed] [Google Scholar]
  18. Lam T. C., Shrive N. G., Frank C. B. Variations in rupture site and surface strains at failure in the maturing rabbit medial collateral ligament. J Biomech Eng. 1995 Nov;117(4):455–461. doi: 10.1115/1.2794207. [DOI] [PubMed] [Google Scholar]
  19. Marinozzi G., Ferrante F., Gaudio E., Ricci A., Amenta F. Intrinsic innervation of the rat knee joint articular capsule and ligaments. Acta Anat (Basel) 1991;141(1):8–14. doi: 10.1159/000147091. [DOI] [PubMed] [Google Scholar]
  20. McDougall J. J., Karimian S. M., Ferrell W. R. Alteration of substance P-mediated vasodilatation and sympathetic vasoconstriction in the rat knee joint by adjuvant-induced inflammation. Neurosci Lett. 1994 Jun 20;174(2):127–129. doi: 10.1016/0304-3940(94)90002-7. [DOI] [PubMed] [Google Scholar]
  21. McDougall J. J., Karimian S. M., Ferrell W. R. Prolonged alteration of vasoconstrictor and vasodilator responses in rat knee joints by adjuvant monoarthritis. Exp Physiol. 1995 May;80(3):349–357. doi: 10.1113/expphysiol.1995.sp003852. [DOI] [PubMed] [Google Scholar]
  22. Najafipour H., Ferrell W. R. Sympathetic innervation and alpha-adrenoceptor profile of blood vessels in the posterior region of the rabbit knee joint. Br J Pharmacol. 1993 Jan;108(1):79–84. doi: 10.1111/j.1476-5381.1993.tb13443.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SKOGLUND S. Anatomical and physiological studies of knee joint innervation in the cat. Acta Physiol Scand Suppl. 1956;36(124):1–101. [PubMed] [Google Scholar]

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