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. 1979 Apr 15;180(1):75–85. doi: 10.1042/bj1800075

Comparison of permeant ion uptake and carotenoid band shift as methods for determining the membrane potential in chromatophores from Rhodopseudomonas sphaeroides Ga.

S J Ferguson, O T Jones, D B Kell, M C Sorgato
PMCID: PMC1161021  PMID: 226068

Abstract

1. A comparison was made of two methods for estimating the membrane potential in chromatophores from Rhodopseudomonas sphaeroides Ga. Illuminated chromatophores generated a potential that is apparently much larger when estimated on the basis of the red-band shift of carotenoids rather than from the extent of uptake of the permeant SCN- ion. 2. In contrast, when the chromatophores were oxidizing NADH or succinate the uptake of SCN- indicated a larger membrane potential than was estimated from the carotenoid band shift. 3. The extent of SCN- uptake and the carotenoid-band shift respond differently to changes in the ionic composition of the reaction medium. 4. The effects of antimycin on the carotenoid band shift and SCN- uptake are reported. 5. It is concluded that the carotenoid band shift and the uptake of SCN- are responding to different aspects of the energized state.

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Selected References

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  1. Baccarini Melandri A., Casadio R., Melandri B. A. Thermodynamics and kinetics of photophosphorylation in bacterial chromatophores and their relation with the transmembrane electrochemical potential difference of protons. Eur J Biochem. 1977 Sep;78(2):389–402. doi: 10.1111/j.1432-1033.1977.tb11751.x. [DOI] [PubMed] [Google Scholar]
  2. Casadio R., Baccarini Melandri A., Zannoni D., Melandri B. A. Electrochemical proton gradient and phosphate potential in bacterial chromatophores. FEBS Lett. 1974 Dec 15;49(2):203–207. doi: 10.1016/0014-5793(74)80512-0. [DOI] [PubMed] [Google Scholar]
  3. Casadio R., Baccarini-Melandri A., Melandri B. A. On the determination of the transmembrane pH difference in bacterial chromatophores using 9-aminoacridine. Eur J Biochem. 1974 Aug 15;47(1):121–128. doi: 10.1111/j.1432-1033.1974.tb03675.x. [DOI] [PubMed] [Google Scholar]
  4. Case G. D., Parson W. W. Shifts of bacteriochlorophyll and carotenoid absorption bands linked to cytochrome c-555 photooxidation in Chromatium. Biochim Biophys Acta. 1973 Dec 14;325(3):441–453. doi: 10.1016/0005-2728(73)90205-3. [DOI] [PubMed] [Google Scholar]
  5. Chance B. Carotenoid and merocyanine probes in chromatophore membranes. Biomembranes. 1975;7:33–55. doi: 10.1007/978-1-4684-7668-2_3. [DOI] [PubMed] [Google Scholar]
  6. Chance B. Electron transfer: pathways, mechanisms, and controls. Annu Rev Biochem. 1977;46:967–980. doi: 10.1146/annurev.bi.46.070177.004535. [DOI] [PubMed] [Google Scholar]
  7. Connelly J. L., Jones O. T., Saunders V. A., Yates D. W. Kinetic and thermodynamic properties of membrane-bound cytochromes of aerobically and photosynthetically grown Rhodopseudomonas spheroides. Biochim Biophys Acta. 1973 Apr 5;292(3):644–653. doi: 10.1016/0005-2728(73)90012-1. [DOI] [PubMed] [Google Scholar]
  8. Jackson J. B., Crofts A. R. The high energy state in chromatophores from Rhodopseudomonas spheroides. FEBS Lett. 1969 Aug;4(3):185–189. doi: 10.1016/0014-5793(69)80230-9. [DOI] [PubMed] [Google Scholar]
  9. Jackson J. B., Crofts A. R. The kinetics of light induced carotenoid changes in Rhodopseudomonas spheroides and their relation to electrical field generation across the chromatophore membrane. Eur J Biochem. 1971 Jan 1;18(1):120–130. doi: 10.1111/j.1432-1033.1971.tb01222.x. [DOI] [PubMed] [Google Scholar]
  10. Jackson J. B., Dutton P. L. The kinetic and redox potentiometric resolution of the carotenoid shifts in Rhodopseudomonas spheroides chromatophores: their relationship to electric field alterations in electron transport and energy coupling. Biochim Biophys Acta. 1973 Oct 19;325(1):102–113. doi: 10.1016/0005-2728(73)90155-2. [DOI] [PubMed] [Google Scholar]
  11. Jackson J. B., Saphon S., Witt H. T. The extent of the stimulated electrical potential decay under phosphorylating conditions and the H+/ATP ratio in Rhodopseudomonas sphaeroides chromatophores following short flash excitation. Biochim Biophys Acta. 1975 Oct 10;408(1):83–92. doi: 10.1016/0005-2728(75)90160-7. [DOI] [PubMed] [Google Scholar]
  12. Kell D. B., Ferguson S. J., John P. Measurement by a flow dialysis technique of the steady-state proton-motive force in chromatophores from Rhodospirillum rubrum. Comparison with phosphorylation potential. Biochim Biophys Acta. 1978 Apr 11;502(1):111–126. doi: 10.1016/0005-2728(78)90136-6. [DOI] [PubMed] [Google Scholar]
  13. Kell D. B., John P., Ferguson S. J. The protonmotive force in phosphorylating membrane vesicles from Paracoccus denitrificans. Magnitude, sites of generation and comparison with the phosphorylation potential. Biochem J. 1978 Jul 15;174(1):257–266. doi: 10.1042/bj1740257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kell D. B., John P., Sorgato M. C., Ferguson S. J. Continuous monitoring of the electrical potential across energy-transducing membranes using ion-selective electrodes. Application to submitochondrial particles and chromatophores. FEBS Lett. 1978 Feb 15;86(2):294–298. doi: 10.1016/0014-5793(78)80583-3. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Michels P. A., Konings W. N. The electrochemical proton gradient generated by light in membrane vesicles and chromatophores from Rhodopseudomonas sphaeroides. Eur J Biochem. 1978 Apr;85(1):147–155. doi: 10.1111/j.1432-1033.1978.tb12222.x. [DOI] [PubMed] [Google Scholar]
  17. Packham N. K., Berriman J. A., Jackson J. B. The charging capacitance of the chromatophore membrane. FEBS Lett. 1978 May 15;89(2):205–210. doi: 10.1016/0014-5793(78)80218-x. [DOI] [PubMed] [Google Scholar]
  18. Prince R. C., Dutton P. L. A kinetic completion of the cyclic photosynthetic electron pathway of Rhodopseudomonas sphaeroides: cytochrome b-cytochrome c2 oxidation-reduction. Biochim Biophys Acta. 1975 Jun 17;387(3):609–613. doi: 10.1016/0005-2728(75)90101-2. [DOI] [PubMed] [Google Scholar]
  19. Rottenberg H., Grunwald T., Avron M. Determination of pH in chloroplasts. I. Distribution of ( 14 C) methylamine. Eur J Biochem. 1972 Jan 31;25(1):54–63. doi: 10.1111/j.1432-1033.1972.tb01666.x. [DOI] [PubMed] [Google Scholar]
  20. Rottenberg H. The measurement of transmembrane electrochemical proton gradients. J Bioenerg. 1975 May;7(2):61–74. doi: 10.1007/BF01558427. [DOI] [PubMed] [Google Scholar]
  21. SISTROM W. R. A requirement for sodium in the growth of Rhodopseudomonas spheroides. J Gen Microbiol. 1960 Jun;22:778–785. doi: 10.1099/00221287-22-3-778. [DOI] [PubMed] [Google Scholar]
  22. SISTROM W. R., CLAYTON R. K. STUDIES ON A MUTANT OF RHODOPSEUDOMONAS SPHEROIDES UNABLE TO GROW PHOTOSYNTHETICALLY. Biochim Biophys Acta. 1964 Jul 29;88:61–73. doi: 10.1016/0926-6577(64)90154-8. [DOI] [PubMed] [Google Scholar]
  23. Schuldiner S., Padan E., Rottenberg H., Gromet-Elhanan Z., Avron M. Delta pH and membrane potential in bacterial chromatophores. FEBS Lett. 1974 Dec 15;49(2):174–177. doi: 10.1016/0014-5793(74)80505-3. [DOI] [PubMed] [Google Scholar]
  24. Sewe K. U., Reich R. Influence of the chlorophylls on the electrochromism of carotenoids in the membranes of photosynthesis. FEBS Lett. 1977 Aug 1;80(1):30–34. doi: 10.1016/0014-5793(77)80400-6. [DOI] [PubMed] [Google Scholar]
  25. Sherman L. A., Clayton R. K. The lack of carotenoid band shifts in a non-photosynthetic, reaction centerless mutant of Rhodopseudomonas spheroides. FEBS Lett. 1972 Apr 15;22(1):127–132. doi: 10.1016/0014-5793(72)80237-0. [DOI] [PubMed] [Google Scholar]
  26. Sorgato M. C., Ferguson S. J., Kell D. B., John P. The protonmotive force in bovine heart submitochondrial particles. Magnitude, sites of generation and comparison with the phosphorylation potential. Biochem J. 1978 Jul 15;174(1):237–256. doi: 10.1042/bj1740237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sorgato M. C., Ferguson S. J. Measurements of the components of the protonmotive force generated by cytochrome oxidase in submitochondrial particles. FEBS Lett. 1978 Jun 1;90(1):178–182. doi: 10.1016/0014-5793(78)80324-x. [DOI] [PubMed] [Google Scholar]
  28. Symons M., Swysen C., Sybesma C. The light-induced carotenoid absorbance changes in Rhodopseudomonas sphaeroides: an analysis and interpretation of the band shifts. Biochim Biophys Acta. 1977 Dec 23;462(3):706–717. doi: 10.1016/0005-2728(77)90112-8. [DOI] [PubMed] [Google Scholar]
  29. Takamiya K., Dutton P. L. The influence of transmembrane potentials of the redox equilibrium between cytochrome c2 and the reaction center in Rhodopseudomonas sphaeroides chromatophores. FEBS Lett. 1977 Aug 15;80(2):279–284. doi: 10.1016/0014-5793(77)80457-2. [DOI] [PubMed] [Google Scholar]
  30. Vredenberg W. J., Tonk W. J. On the steady-state electrical potential difference across the thylakoid membranes of chloroplasts in illuminated plant cells. Biochim Biophys Acta. 1975 Jun 17;387(3):580–587. doi: 10.1016/0005-2728(75)90095-x. [DOI] [PubMed] [Google Scholar]
  31. Young J. H. Light induced dipole layers in the thylakoid membrane. J Theor Biol. 1974 Feb;43(2):339–350. doi: 10.1016/s0022-5193(74)80065-2. [DOI] [PubMed] [Google Scholar]

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