Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1979 May 15;180(2):427–429. doi: 10.1042/bj1800427

The effect of surgical trauma on muscle protein turnover in rats. A serious methodological misunderstanding.

D J Millward
PMCID: PMC1161068  PMID: 486118

Abstract

The reported rates of protein degradation in a recent paper on the effect of surgical trauma on muscle protein turnover [Hoover-Plow & Clifford (1978) Biochem. J. 176, 137--142] have no real meaning because of a serious methodological misunderstanding by the authors. In addition, there are problems involved in the determination of synthesis rates, so that the reported effects of trauma on muscle protein turnover can be discounted.

Full text

PDF
427

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Garlick P. J., Millward D. J. An appraisal of techniques for the determination of protein turnover in vivo. Proc Nutr Soc. 1972 Dec;31(3):249–255. doi: 10.1079/pns19720048. [DOI] [PubMed] [Google Scholar]
  2. Garlick P. J., Waterlow J. C., Swick R. W. Measurement of protein turnover in rat liver. Analysis of the complex curve for decay of label in a mixture of proteins. Biochem J. 1976 Jun 15;156(3):657–663. doi: 10.1042/bj1560657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hoover-Plow J. L., Clifford A. J. The effect of surgical trauma on muscle protein turnover in rats. Biochem J. 1978 Oct 15;176(1):137–142. doi: 10.1042/bj1760137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Millward D. J. Protein turnover in skeletal muscle. I. The measurement of rates of synthesis and catabolism of skeletal muscle protein using (14C)Na2CO3 to label protein. Clin Sci. 1970 Nov;39(5):577–590. doi: 10.1042/cs0390577. [DOI] [PubMed] [Google Scholar]
  5. Millward D. J. Protein turnover in skeletal muscle. II. The effect of starvation and a protein-free diet on the synthesis and catabolism of skeletal muscle proteins in comparison to liver. Clin Sci. 1970 Nov;39(5):591–603. doi: 10.1042/cs0390591. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES