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. 1979 May 15;180(2):445–448. doi: 10.1042/bj1800445

The rapid preparation of peroxisomes from rat liver by using a vertical rotor.

C E Neat, H Osmundsen
PMCID: PMC1161073  PMID: 486122

Abstract

A method is described for the rapid preparation of peroxisomes from rat liver by using sucrose-density-gradient centrifugation in a vertical rotor. The preparation, shown to be virtually free of mitochondrial and microsomal contamination, can be used to study fatty acid metabolism by isolated peroxisomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Arif A., Blecher M. Synthesis of fatty acyl CoA and other thiol esters using N-hydroxysuccinimide esters of fatty acids. J Lipid Res. 1969 May;10(3):344–345. [PubMed] [Google Scholar]
  2. Baudhuin P., Beaufay H., Rahman-Li Y., Sellinger O. Z., Wattiaux R., Jacques P., De Duve C. Tissue fractionation studies. 17. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, alanine aminotransferase, D-amino acid oxidase and catalase in rat-liver tissue. Biochem J. 1964 Jul;92(1):179–184. doi: 10.1042/bj0920179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baudhuin P. Isolation of rat liver peroxisomes. Methods Enzymol. 1974;31:356–368. doi: 10.1016/0076-6879(74)31039-7. [DOI] [PubMed] [Google Scholar]
  4. Blouin A., Bolender R. P., Weibel E. R. Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study. J Cell Biol. 1977 Feb;72(2):441–455. doi: 10.1083/jcb.72.2.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Huang A. H., Beevers H. Localization of enzymes within microbodies. J Cell Biol. 1973 Aug;58(2):379–389. doi: 10.1083/jcb.58.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Krahling J. B., Gee R., Murphy P. A., Kirk J. R., Tolbert N. E. Comparison of fatty acid oxidation in mitochondria and peroxisomes from rat liver. Biochem Biophys Res Commun. 1978 May 15;82(1):136–141. doi: 10.1016/0006-291x(78)90587-9. [DOI] [PubMed] [Google Scholar]
  8. Lazarow P. B., De Duve C. A fatty acyl-CoA oxidizing system in rat liver peroxisomes; enhancement by clofibrate, a hypolipidemic drug. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2043–2046. doi: 10.1073/pnas.73.6.2043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leighton F., Poole B., Beaufay H., Baudhuin P., Coffey J. W., Fowler S., De Duve C. The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions. J Cell Biol. 1968 May;37(2):482–513. doi: 10.1083/jcb.37.2.482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pullman M. E. A convenient and versatile method for the purification of CoA thiol esters. Anal Biochem. 1973 Jul;54(1):188–198. doi: 10.1016/0003-2697(73)90262-5. [DOI] [PubMed] [Google Scholar]
  11. SAWANT P. L., SHIBKO, KUMTA U. S., TAPPEL A. L. ISOLATION OF RAT-LIVER LYSOSOMES AND THEIR GENERAL PROPERTIES. Biochim Biophys Acta. 1964 Apr 6;85:82–92. doi: 10.1016/0926-6569(64)90169-5. [DOI] [PubMed] [Google Scholar]
  12. Sottocasa G. L., Kuylenstierna B., Ernster L., Bergstrand A. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol. 1967 Feb;32(2):415–438. doi: 10.1083/jcb.32.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

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