Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1979 Jun 15;180(3):523–531. doi: 10.1042/bj1800523

Acute regulation of pyruvate kinase activity in rat epididymal adipose tissue by insulin.

R M Denton, N J Edgell, B J Bridges, G P Poole
PMCID: PMC1161090  PMID: 486131

Abstract

1. Evidence is presented that exposure of epididymal fat-pads from fed rats to insulin leads to a marked diminution in the Km for phosphoenolpyruvate of pyruvate kinase. Effects of insulin may be readily demonstrated in experiments both in vivo and in vitro and are not secondary to the activation by the hormone of glucose transport. No effect of insulin is apparent in tissues from 48 h-starved animals. 2. The mechanism of the effect of insulin on pyruvate kinase was not established. The observed changes in Km do not appear to be the result of alterations in the amounts of bound effectors such as fructose 1,6-bisphosphate and alanine. Rather, as the effect persists in incubated extracts, it appears that a change in the degree of phosphorylation or some other covalent modification of the enzyme may be involved.

Full text

PDF
523

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berglund L., Ljungström O., Engström L. Purification and characterization of pig kidney pyruvate kinase (type A). J Biol Chem. 1977 Sep 10;252(17):6108–6111. [PubMed] [Google Scholar]
  2. Coore H. G., Denton R. M., Martin B. R., Randle P. J. Regulation of adipose tissue pyruvate dehydrogenase by insulin and other hormones. Biochem J. 1971 Nov;125(1):115–127. doi: 10.1042/bj1250115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Denton R., Bridges B., Brownsey R., Evans G., Hughes W., Stansbie D. Regulation of the conversion of glucose into fat in white adipose tissue by insulin [proceedings]. Biochem Soc Trans. 1977;5(4):894–900. doi: 10.1042/bst0050894. [DOI] [PubMed] [Google Scholar]
  4. Eigenbrodt E., Schoner W. Modification of interconversion of pyruvate kinase type M2 from chicken liver by fructose 1,6-bisphosphate and l-alanine. Hoppe Seylers Z Physiol Chem. 1977 Aug;358(8):1057–1067. doi: 10.1515/bchm2.1977.358.2.1057. [DOI] [PubMed] [Google Scholar]
  5. Eigenbrodt E., Schoner W. Purification and properties of the pyruvate kinase isoenzymes type L and M2 from chicken liver. Hoppe Seylers Z Physiol Chem. 1977 Aug;358(8):1033–1046. doi: 10.1515/bchm2.1977.358.2.1033. [DOI] [PubMed] [Google Scholar]
  6. Engström L. The regulation of liver pyruvate kinase by phosphorylation--dephosphorylation. Curr Top Cell Regul. 1978;13:28–51. [PubMed] [Google Scholar]
  7. Felíu J. E., Díaz-Gil J., García-Cañero R., Gosálvez M. Interconvertible forms of class A pyruvate kinase from Ehrlich ascites tumour cells. FEBS Lett. 1975 Feb 15;50(3):334–338. doi: 10.1016/0014-5793(75)80522-9. [DOI] [PubMed] [Google Scholar]
  8. Felíu J. E., Hue L., Hers H. G. Regulation in vitro and in vivo of adenosine 3':5'-monophosphate-dependent inactivation of rat-liver pyruvate kinase type L. Eur J Biochem. 1977 Dec;81(3):609–617. doi: 10.1111/j.1432-1033.1977.tb11988.x. [DOI] [PubMed] [Google Scholar]
  9. Harada K., Saheki S., Wada K., Tanaka T. Purification of four pyruvate kinase isozymes of rats by affinity elution chromatography. Biochim Biophys Acta. 1978 Jun 9;524(2):327–339. doi: 10.1016/0005-2744(78)90169-9. [DOI] [PubMed] [Google Scholar]
  10. Ibsen K. H., Trippet P. Interconvertible and noninterconvertible forms of rat pyruvate kinase. Biochemistry. 1972 Nov 21;11(24):4442–4450. doi: 10.1021/bi00774a004. [DOI] [PubMed] [Google Scholar]
  11. Imamura K., Tanaka T. Multimolecular forms of pyruvate kinase from rat and other mammalian tissues. I. Electrophoretic studies. J Biochem. 1972 Jun;71(6):1043–1051. doi: 10.1093/oxfordjournals.jbchem.a129852. [DOI] [PubMed] [Google Scholar]
  12. Ljungström O., Ekman P. Glucagon-induced phosphorylation of pyruvate kinase (type L) in rat liver slices. Biochem Biophys Res Commun. 1977 Oct 24;78(4):1147–1155. doi: 10.1016/0006-291x(77)91413-9. [DOI] [PubMed] [Google Scholar]
  13. McCormack J. G., Denton R. M. Evidence that fatty acid synthesis in the interscapular brown adipose tissue of cold-adapted rats is increased in vivo by insulin by mechanisms involving parallel activation of pyruvate dehydrogenase and acetyl-coenzyme A carboxylase. Biochem J. 1977 Sep 15;166(3):627–630. doi: 10.1042/bj1660627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nishimura J., Hamasaki N., Minakami S. Platelet pyruvate kinase. Two interconvertible forms of the enzyme. Biochim Biophys Acta. 1978 Jan 12;522(1):104–112. doi: 10.1016/0005-2744(78)90326-1. [DOI] [PubMed] [Google Scholar]
  15. Pogson C. I. Adipose-tissue pyruvate kinase. Properties and interconversion of two active forms. Biochem J. 1968 Nov;110(1):67–77. doi: 10.1042/bj1100067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pogson C. I. Two interconvertible forms of pyruvate kinase in adipose tissue. Biochem Biophys Res Commun. 1968 Feb 15;30(3):297–302. doi: 10.1016/0006-291x(68)90450-6. [DOI] [PubMed] [Google Scholar]
  17. ROBINSON B. H., WRIGHT P. H. Guinea-pig anti-insulin serum. J Physiol. 1961 Feb;155:302–310. doi: 10.1113/jphysiol.1961.sp006628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Riou J. P., Claus T. H., Pilkis S. J. Stimulation of glucagon of in vivo phosphorylation of rat hepatic pyruvate kinase. J Biol Chem. 1978 Feb 10;253(3):656–659. [PubMed] [Google Scholar]
  19. Schulz J., Sparmann G., Hofmann E. Alanine-mediated reversible inactivation of tumour pyruvate kinase caused by a tetramer-dimer transition. FEBS Lett. 1975 Feb 15;50(3):346–350. doi: 10.1016/0014-5793(75)80524-2. [DOI] [PubMed] [Google Scholar]
  20. Sparmann G., Schulz J., Hofmann E. Effects of L-alanine and fructose (1,6-diphosphate) on pyruvate kinase from ehrlich ascites tumour cells. FEBS Lett. 1973 Nov 1;36(3):305–308. doi: 10.1016/0014-5793(73)80397-7. [DOI] [PubMed] [Google Scholar]
  21. Stansbie D., Brownsey R. W., Crettaz M., Denton R. M. Acute effects in vivo of anti-insulin serum on rates of fatty acid synthesis and activities of acetyl-coenzyme A carboxylase and pyruvate dehydrogenase in liver and epididymal adipose tissue of fed rats. Biochem J. 1976 Nov 15;160(2):413–416. doi: 10.1042/bj1600413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Walker P. R., Potter V. R. Allosteric properties of a pyruvate kinase isoenzyme from rat liver cells in culture. J Biol Chem. 1973 Jul 10;248(13):4610–4616. [PubMed] [Google Scholar]
  23. van Berkel T. J., de Jonge H. R., Koster J. F., Hülsmann W. C. Kinetic evidence for the presence of two forms of M2-type pyruvate kinase in rat small intestine. Biochem Biophys Res Commun. 1974 Sep 9;60(1):398–405. doi: 10.1016/0006-291x(74)90218-6. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES