Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1979 Aug 1;181(2):267–274. doi: 10.1042/bj1810267

Medium-chain fatty acid synthesis in lactating-rabbit mammary gland. Intracellular concentration and specificity of medium-chain acyl thioester hydrolase.

J Knudsen
PMCID: PMC1161158  PMID: 574008

Abstract

The concentration of medium-chain acyl thioester hydrolase and of fatty acid synthetase was determined by rocket immunoelectrophoresis in nine different particle-free supernatant fractions from lactating-rabbit mammary gland. The molar ratio of the hydrolase to fatty acid synthetase was 1.99 +/- 0.66 (mean +/- S.D.). A rate-limiting concentration of malonyl-CoA was required to ensure the predominant synthesis of medium-chain fatty acids when 2 mol of the hydrolase was added per mol of fatty acid synthetase. The interaction of the hydrolase with fatty acid synthetase was concentration-dependent, though an optimum concentration of hydrolase to synthetase could not be obtained. The lactating-rabbit mammary gland hydrolase altered the pattern of fatty acids synthesized by fatty acid synthetases prepared from cow, goat, sheep and rabbit lactating mammary glands, rabbit liver and cow adipose tissue.

Full text

PDF
267

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carey E. M., Dils R. The pattern of fatty acid synthesis in lactating rabbit mammary gland studied in vivo. Biochem J. 1972 Feb;126(4):1005–1007. doi: 10.1042/bj1261005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chivers L., Knudsen J., Dils R. Immunological properties of medium-chain acyl-thioester hydrolase and fatty acid synthetase from lactating-rabbit mammary gland. Biochim Biophys Acta. 1977 May 25;487(2):361–367. doi: 10.1016/0005-2760(77)90012-1. [DOI] [PubMed] [Google Scholar]
  3. Cook G. A., Nielsen R. C., Hawkins R. A., Mehlman M. A., Lakshmanan M. R., Veech R. L. Effect of glucagon on hepatic malonyl coenzyme A concentration and on lipid synthesis. J Biol Chem. 1977 Jun 25;252(12):4421–4424. [PubMed] [Google Scholar]
  4. EGGERER H., LYNEN F. [On the biosynthesis of fatty acids. II. Synthesis and properties of S-malonyl-coenzyme A]. Biochem Z. 1962;335:540–547. [PubMed] [Google Scholar]
  5. Grunnet I., Knudsen J. Molecular weight and subunit size of rabbit mammary-gland fatty acid synthetase. Biochem J. 1978 Sep 1;173(3):929–933. doi: 10.1042/bj1730929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guynn R. W., Veloso D., Veech R. L. The concentration of malonyl-coenzyme A and the control of fatty acid synthesis in vivo. J Biol Chem. 1972 Nov 25;247(22):7325–7331. [PubMed] [Google Scholar]
  7. Harboe N., Ingild A. Immunization, isolation of immunoglobulins, estimation of antibody titre. Scand J Immunol Suppl. 1973;1:161–164. doi: 10.1111/j.1365-3083.1973.tb03798.x. [DOI] [PubMed] [Google Scholar]
  8. Hsu R. Y., Wasson G., Porter J. W. The purification and properties of the fatty acid synthetase of pigeon liver. J Biol Chem. 1965 Oct;240(10):3736–3746. [PubMed] [Google Scholar]
  9. Knudsen J., Clark S., Dils R. Purification and some properties of a medium-chain acyl-thioester hydrolase from lactating-rabbit mammary gland which terminates chain elongation in fatty acid synthesis. Biochem J. 1976 Dec 15;160(3):683–691. doi: 10.1042/bj1600683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Knudsen J. Fatty acid synthetase from cow mammary gland tissue cells. Biochim Biophys Acta. 1972 Nov 30;280(3):408–414. doi: 10.1016/0005-2760(72)90246-9. [DOI] [PubMed] [Google Scholar]
  11. Knudsen J. The role of particulate esterification in chain length control of de novo synthesized fatty acids in liver and mammary gland. Comp Biochem Physiol B. 1976;53(1):3–7. doi: 10.1016/0305-0491(76)90086-9. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Libertini L. J., Smith S. Purification and properties of a thioesterase from lactating rat mammary gland which modifies the product specificity of fatty acid synthetase. J Biol Chem. 1978 Mar 10;253(5):1393–1401. [PubMed] [Google Scholar]
  14. Numa S. Regulation of fatty-acid synthesis in higher animals. Ergeb Physiol. 1974;69(0):54–96. [PubMed] [Google Scholar]
  15. Sumper M., Oesterhelt D., Riepertinger C., Lynen F. Die Synthese verschiedener Carbonsäuren durch den Multienzymkomplex der Fettsäuresynthese aus Hefe und die Erklärung ihrer Bildung. Eur J Biochem. 1969 Sep;10(2):377–387. doi: 10.1111/j.1432-1033.1969.tb00701.x. [DOI] [PubMed] [Google Scholar]
  16. Sánchez M., Nicholls D. G., Brindley D. N. [The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate in rat liver mitochondria]. Biochem J. 1973 Apr;132(4):697–706. doi: 10.1042/bj1320697. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES