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. 1979 Sep 1;181(3):759–761. doi: 10.1042/bj1810759

Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.

R Lichtner, H U Wolf
PMCID: PMC1161216  PMID: 42388

Abstract

A simple method is described for dodecyl sulphate/polyacrylamide-gel electrophoresis of pH- and temperature-labile biological intermediates. The method is based on a catalyst system that works at temperatures of 2--4 degrees C and pH values of 2--4 and an appropriate buffer system containing Li+ or Tris [CH2OH--C(CH2OH)2--NH3+] instead of Na+. This system does not lead to the precipitation of 1% dodecyl sulphate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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