Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1979 Sep 1;181(3):763–766. doi: 10.1042/bj1810763

The oxidation-reduction potentials of cytochrome o + c4 and cytochrome o purified from Azotobacter vinelandii.

T Yang, D O'Keefe, B Chance
PMCID: PMC1161217  PMID: 518554

Abstract

Oxidation-reduction titrations of Azotobacter vinelandii cytochrome o + c4 and cytochrome o were performed with simultaneous potential and absorbance measurements under anaerobic conditions. Cytochrome c4 has a midpoint potential (Em, 7.4) of 260mV and purified cytochrome o has an Em, 7.4 of -18mV. Little change in the midpoint potential of cytochrome o was observed when titrated in the pH range 6.2--9.8.

Full text

PDF
763

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CASTOR L. N., CHANCE B. Photochemical determinations of the oxidases of bacteria. J Biol Chem. 1959 Jun;234(6):1587–1592. [PubMed] [Google Scholar]
  2. CHANCE B., SMITH L., CASTOR L. New methods for the study of the carbon monoxide compounds of respiratory enzymes. Biochim Biophys Acta. 1953 Sep-Oct;12(1-2):289–298. doi: 10.1016/0006-3002(53)90148-6. [DOI] [PubMed] [Google Scholar]
  3. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  4. Dutton P. L. Oxidation-reduction potential dependence of the interaction of cytochromes, bacteriochlorophyll and carotenoids at 77 degrees K in chromatophores of Chromatium D and Rhodopseudomonas gelatinosa. Biochim Biophys Acta. 1971 Jan 12;226(1):63–80. doi: 10.1016/0005-2728(71)90178-2. [DOI] [PubMed] [Google Scholar]
  5. Jones C. W., Redfearn E. R. The cytochrome system of Azotobacter vinelandii. Biochim Biophys Acta. 1967 Sep 6;143(2):340–353. doi: 10.1016/0005-2728(67)90088-6. [DOI] [PubMed] [Google Scholar]
  6. Kauffman H. F., van Gelder B. F. The respiratory chain of Azotobacter vinelandii. I. Spectral properites of cytochrome d. Biochim Biophys Acta. 1973 May 30;305(2):260–267. doi: 10.1016/0005-2728(73)90174-6. [DOI] [PubMed] [Google Scholar]
  7. Swank R. T., Burris R. H. Purification and properties of cytochromes c of Azotobacter vinelandii. Biochim Biophys Acta. 1969 Aug 5;180(3):473–489. doi: 10.1016/0005-2728(69)90026-7. [DOI] [PubMed] [Google Scholar]
  8. Tyree B., Webster D. A. Electron-accepting properties of cytochrome o purified from Vitreoscilla. J Biol Chem. 1978 Nov 10;253(21):7635–7637. [PubMed] [Google Scholar]
  9. Tyree B., Webster D. A. The binding of cyanide and carbon monoxide to cytochrome o purified from Vitreoscilla. Evidence for subunit interaction in the reduced protein. J Biol Chem. 1978 Oct 10;253(19):6988–6991. [PubMed] [Google Scholar]
  10. Webster D. A., Hackett D. P. The purification and properties of cytochrome o from Vitreoscilla. J Biol Chem. 1966 Jul 25;241(14):3308–3315. [PubMed] [Google Scholar]
  11. Yamanaka T., Imai S. A cytochrome cc'-like haemoprotein isolated from Azotobacter vinelandii. Biochem Biophys Res Commun. 1972 Jan 14;46(1):150–154. doi: 10.1016/0006-291x(72)90643-2. [DOI] [PubMed] [Google Scholar]
  12. Yang T. Y., Jurtshuk P., Jr Purification and characterization of cytochrome O from Azotobacter vinelandii. Biochim Biophys Acta. 1978 Jun 8;502(3):543–548. doi: 10.1016/0005-2728(78)90086-5. [DOI] [PubMed] [Google Scholar]
  13. Yang T. Y., Jurtshuk P., Jr Studies on the red oxidase (cytochrome o) of Azotobacter vinelandii. Biochem Biophys Res Commun. 1978 Apr 14;81(3):1032–1039. doi: 10.1016/0006-291x(78)91454-7. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES