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. 1980 Jan 1;185(1):59–63. doi: 10.1042/bj1850059

The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica.

S G Hughes, T Bruce, K Murray
PMCID: PMC1161269  PMID: 6246880

Abstract

An endonuclease, AsuI, was isolated from extracts of Anabaena subcylindrica on the basis of gel-electrophoretic analysis of digests of bacteriophage-lambda DNA with the paritally purified extracts. The enzyme requires Mg2+, but no other cofactors. Endonuclease AsuI recognizes the interrupted tetranucleotide sequence: (Formula: see tex), and breaks the phosphodiester bonds indicated by the arrows to leave single-stranded trinucleotide projections at the 5'-termini of the DNA fragments.

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Selected References

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  1. Allen M. B., Arnon D. I. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm. Plant Physiol. 1955 Jul;30(4):366–372. doi: 10.1104/pp.30.4.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bigger C. H., Murray K., Murray N. E. Recognition sequence of a restriction enzyme. Nat New Biol. 1973 Jul 4;244(131):7–10. doi: 10.1038/newbio244007a0. [DOI] [PubMed] [Google Scholar]
  3. Bron S., Murray K. Restriction and modification in B. subtilis. Nucleotide sequence recognised by restriction endonuclease R. Bsu R from strain R. Mol Gen Genet. 1975 Dec 30;143(1):25–33. doi: 10.1007/BF00269417. [DOI] [PubMed] [Google Scholar]
  4. Hedgpeth J., Goodman H. M., Boyer H. W. DNA nucleotide sequence restricted by the RI endonuclease. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3448–3452. doi: 10.1073/pnas.69.11.3448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kelly T. J., Jr, Smith H. O. A restriction enzyme from Hemophilus influenzae. II. J Mol Biol. 1970 Jul 28;51(2):393–409. doi: 10.1016/0022-2836(70)90150-6. [DOI] [PubMed] [Google Scholar]
  6. Murray K., Hughes S. G., Brown J. S., Bruce S. A. Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis. Biochem J. 1976 Nov;159(2):317–322. doi: 10.1042/bj1590317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Murray K. Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424. Biochem J. 1973 Mar;131(3):569–582. doi: 10.1042/bj1310569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Richardson C. C. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158–165. doi: 10.1073/pnas.54.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  10. Smith H. O., Nathans D. Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J Mol Biol. 1973 Dec 15;81(3):419–423. doi: 10.1016/0022-2836(73)90152-6. [DOI] [PubMed] [Google Scholar]
  11. Smith H. O., Wilcox K. W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970 Jul 28;51(2):379–391. doi: 10.1016/0022-2836(70)90149-x. [DOI] [PubMed] [Google Scholar]
  12. Sussenbach J. S., Steenbergh P. H., Rost J. A., van Leeuwen W. J., van Embden J. D. A second site-specific restriction endonuclease from Staphylococcus aureus. Nucleic Acids Res. 1978 Apr;5(4):1153–1163. doi: 10.1093/nar/5.4.1153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Thompson R., Hughes S. G., Broda P. Plasmid identification using specific endonucleases. Mol Gen Genet. 1974;133(2):141–149. doi: 10.1007/BF00264835. [DOI] [PubMed] [Google Scholar]
  14. de Waard A., Korsuize J., van Beveren C. P., Maat J. A new sequence-specific endonuclease from Anabaena cylindrica. FEBS Lett. 1978 Dec 1;96(1):106–110. doi: 10.1016/0014-5793(78)81072-2. [DOI] [PubMed] [Google Scholar]
  15. de Waard A., van Beveren C. P., Duyvesteyn M., van Ormondt H. Two sequence-specific endonucleases from Anabaena oscillariodes. FEBS Lett. 1979 May 1;101(1):71–76. [PubMed] [Google Scholar]

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