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. 1980 Jan 1;185(1):253–257. doi: 10.1042/bj1850253

Labelling of the cyclitol permease in Klebsiella aerogenes.

G Reber, M Ropars, J Deshusses
PMCID: PMC1161292  PMID: 6990918

Abstract

The protein component(s) of the cyclitol-transport system in Klebsiella aerogenes has been labelled by using three different procedures. One method is based on differential alkylation with N-ethylmaleimide, and another on incorporation of amino acids during the induction process. A protein of mol.wt. 34 000 was labelled by both procedures; by alkylation with N-ethylmaleimide two other proteins of mol.wts. 55 000 and 67 000 were also labelled. The third uses diazotized [35S]sulphanilic acid after protection by substrate and the comparison of labelling of induced cells with non-induced cells; the label was also concentrated in a mol.wt.-33 000 peak. The labelled protein is, from the evidence, the cyclitol carrier.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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