Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1980 Jan 1;185(1):285–287. doi: 10.1042/bj1850285

Haemoglobin binding with haptoglobin. Unequivocal demonstration that the beta-chains of human haemoglobin bind to haptoglobin.

A L Kazim, M Z Atassi
PMCID: PMC1161299  PMID: 7378053

Abstract

Haptoglobin binding to haemoglobin and its isolated alpha- and beta-chains was studied by use of a highly sensitive solid-phase radiometric assay. As expected, adsorbents of haemoglobin bound 125I-labelled haptoglobin more efficiently than did adsorbents of the alpha-chain. However, unexpectedly, adsorbents of the beta-chain were found to be essentially identical with those of the alpha-chain in their ability to bind haptoglobin. These results demonstrate, unequivocally, the ability of beta-chains to bind to haptoglobin, and indicate that this assay is particularly convenient and useful for studying haptoglobin interactions with haemoglobin and its alpha- and beta-chains.

Full text

PDF
285

Images in this article

286Fig. 1.
on p.286

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atassi M. Z. Chemical studies on haemoglobins A1 and A0. Biochem J. 1964 Oct;93(1):189–197. doi: 10.1042/bj0930189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atassi M. Z., Sakata S., Kazim A. L. Localization and verification by synthesis of five antigenic sites of bovine serum albumin. Biochem J. 1979 May 1;179(2):327–331. doi: 10.1042/bj1790327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chiancone E., Alfsen A., Ioppolo C., Vecchini P., Agrò A. F., Wyman J., Antonini E. Studies on the reaction of haptoglobin with haemoglobin and haemoglobin chains. I. Stoichiometry and affinity. J Mol Biol. 1968 Jul 14;34(2):347–356. doi: 10.1016/0022-2836(68)90258-1. [DOI] [PubMed] [Google Scholar]
  4. Geraci G., Parkhurst L. J., Gibson Q. H. Preparation and properties of alpha- and beta-chains from human hemoglobin. J Biol Chem. 1969 Sep 10;244(17):4664–4667. [PubMed] [Google Scholar]
  5. Gray G. D., Mickelson M. M., Crim J. A. The demonstration of two gamma-globulin subclasses in the goat. Immunochemistry. 1969 Sep;6(5):641–644. doi: 10.1016/0019-2791(67)90128-0. [DOI] [PubMed] [Google Scholar]
  6. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  7. Kazim A. L., Atassi M. Z. Prediction and conformation by synthesis of two antigenic sites in human haemoglobin by extrapolation from the known antigenic structure of sperm-whale myoglobin. Biochem J. 1977 Oct 1;167(1):275–278. doi: 10.1042/bj1670275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee C. L., Atassi M. Z. Enzymic and immunochemical properties of lysozyme. Accurate definition of the antigenic site around the disulphide bridge 30-115 (site 3) by 'surface-simulation' synthesis. Biochem J. 1977 Dec 1;167(3):571–581. doi: 10.1042/bj1670571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lee C. L., Atassi M. Z. Enzymic and immunochemical properties of lysozyme. XIX. Definition of the direction and conformational restrictions of the antigenic site around the disulfide bonds 64-80 and 76-94 (site 2) by 'surface-simulation' synthesis. Biochim Biophys Acta. 1977 Dec 20;495(2):354–368. doi: 10.1016/0005-2795(77)90391-9. [DOI] [PubMed] [Google Scholar]
  10. March S. C., Parikh I., Cuatrecasas P. A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal Biochem. 1974 Jul;60(1):149–152. doi: 10.1016/0003-2697(74)90139-0. [DOI] [PubMed] [Google Scholar]
  11. Waks M., Alfsen A. Structural studies of haptoglobins. I. Hydrogen ion equilibria and optical rotatory dispersion of Hp 1-1 and Hp 2-2. Arch Biochem Biophys. 1966 Feb;113(2):304–314. doi: 10.1016/0003-9861(66)90191-3. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES