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. 2024 Dec 3;12:RP87120. doi: 10.7554/eLife.87120

Figure 3. Acute microglial metabolic response (aMMR) is required for diet-induced homeostatic rewiring in vivo.

(A) Immunostaining of TOMM20 and DRP1 on sorted microglia from mice Drp1MGWT or Drp1MGKO. (B) Western blot against DRP1 on sorted microglia from mice Drp1MGWT or Drp1MGKO. (C) Graphs showing the overnight fasted glycemia, the 2 hr fasted glycemia, and the insulin released from Drp1MGWT or Drp1MGKO fed with control diet (n=5–11). (D) Graphs showing the overnight fasted glycemia, the 2 hr fasted glycemia, and the insulin released from Drp1MGWT or Drp1MGKO fed with 3-day high-fat diet (n=5–11). Data are presented as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 as determined by two-tailed Student’s t-test and two-way ANOVA followed by Bonferroni post hoc test.

Figure 3—source data 1. Original uncropped image for Figure 3B.
Figure 3—source data 2. Labelled uncropped image for Figure 3B.

Figure 3.

Figure 3—figure supplement 1. Acute microglial metabolic response (aMMR) is required for diet-induced homeostatic rewiring in vivo (supplement).

Figure 3—figure supplement 1.

(A) Schematic depicting the timeline for the tamoxifen injection and the experiments performed on Drp1MGWT or Drp1MGKO. (B) Graph showing the body weight evolution among all the Drp1MGKO genotypes after the tamoxifen injection (n=6–11). (C) Graphs showing the fat mass and lean mass evolution among all the Drp1MGKO genotypes after the tamoxifen injection (n=6–11). (D) Graphs showing the body weight before and after the 3-day high-fat diet (HFD) for the mice Drp1MGWT and Drp1MGKO (n=11–13). Data are presented as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 as determined by two-tailed Student’s t-test and two-way ANOVA followed by Bonferroni post hoc.