Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1980 Mar 1;185(3):689–693. doi: 10.1042/bj1850689

Properties of freshly purified and thiol-treated spinach chloroplast fructose bisphosphatase.

S A Charles, B Halliwell
PMCID: PMC1161446  PMID: 6248029

Abstract

Freshly purified spinach chloroplast fructose bisphosphatase is powerfully inhibited by inorganic phosphate competitively with respect to its substrate fructose 1,6-bisphosphate. The concentrations of phosphate and substrate in the chloroplast stroma are such that the enzyme in this form could not operate at a significant rate in vivo. Incubation of the enzyme with dithiothreitol for 24 h decreases the Km for fructose 1,6-bisphosphate from 0.8 to 0.033 mM, decreases the Km for Mg2+ from 9 to 2 mM and substantially alleviates inhibition by inorganic phosphate. The physiological significance of thiol activation of the enzyme is discussed.

Full text

PDF
689

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson L. E., Avron M. Light Modulation of Enzyme Activity in Chloroplasts: Generation of Membrane-bound Vicinal-Dithiol Groups by Photosynthetic Electron Transport. Plant Physiol. 1976 Feb;57(2):209–213. doi: 10.1104/pp.57.2.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baier D., Latzko E. Properties and regulation of C-1-fructose-1,6-diphosphatase from spinach chloroplasts. Biochim Biophys Acta. 1975 Jul 8;396(1):141–148. doi: 10.1016/0005-2728(75)90197-8. [DOI] [PubMed] [Google Scholar]
  3. Buchanan B. B., Schürmann P., Kalberer P. P. Ferredoxin-activated fructose diphosphatase of spinach chloroplasts. Resolution of the system, properties of the alkaline fructose diphosphatase component, and physiological significance of the ferredoxin-linked activation. J Biol Chem. 1971 Oct 10;246(19):5952–5959. [PubMed] [Google Scholar]
  4. Halliwell B. The chloroplast at work. A review of modern developments in our understanding of chloroplast metabolism. Prog Biophys Mol Biol. 1978;33(1):1–54. doi: 10.1016/0079-6107(79)90024-5. [DOI] [PubMed] [Google Scholar]
  5. Kaiser W. M., Bassham J. A. Light-Dark Regulation of Starch Metabolism in Chloroplasts: I. Levels of Metabolites in Chloroplasts and Medium during Light-Dark Transition. Plant Physiol. 1979 Jan;63(1):105–108. doi: 10.1104/pp.63.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kelly G. J., Zimmermann G., Latzko E. Light induced activation of fructose-1, 6-bisphosphatase in isolated intact chloroplasts. Biochem Biophys Res Commun. 1976 May 3;70(1):193–199. doi: 10.1016/0006-291x(76)91127-x. [DOI] [PubMed] [Google Scholar]
  7. Krause G. H. Light-induced movement of magnesium ions in intact chloroplasts. Spectroscopic determination with Eriochrome Blue SE. Biochim Biophys Acta. 1977 Jun 9;460(3):500–510. doi: 10.1016/0005-2728(77)90088-3. [DOI] [PubMed] [Google Scholar]
  8. Lilley R. M., Chon C. J., Mosbach A., Heldt H. W. The distribution of metabolites between spinach chloroplasts and medium during photosynthesis in vitro. Biochim Biophys Acta. 1977 May 11;460(2):259–272. doi: 10.1016/0005-2728(77)90212-2. [DOI] [PubMed] [Google Scholar]
  9. Portis A. R., Jr, Heldt H. W. Light-dependent changes of the Mg2+ concentration in the stroma in relation to the Mg2+ dependency of CO2 fixation in intact chloroplasts. Biochim Biophys Acta. 1976 Dec 6;449(3):434–436. doi: 10.1016/0005-2728(76)90154-7. [DOI] [PubMed] [Google Scholar]
  10. RACKER E., SCHROEDER E. A. The reductive pentose phosphate cycle. II. Specific C-1 phosphatases for fructose 1,6-diphosphate and sedoheptulose 1,7-diphosphate. Arch Biochem Biophys. 1958 Apr;74(2):326–344. doi: 10.1016/0003-9861(58)90004-3. [DOI] [PubMed] [Google Scholar]
  11. Schürmann P., Wolosiuk R. A. Studies on the regulatory properties of chloroplast fructose-1,6-bisphosphatase. Biochim Biophys Acta. 1978 Jan 12;522(1):130–138. doi: 10.1016/0005-2744(78)90329-7. [DOI] [PubMed] [Google Scholar]
  12. Werdan K., Heldt H. W., Milovancev M. The role of pH in the regulation of carbon fixation in the chloroplast stroma. Studies on CO2 fixation in the light and dark. Biochim Biophys Acta. 1975 Aug 11;396(2):276–292. doi: 10.1016/0005-2728(75)90041-9. [DOI] [PubMed] [Google Scholar]
  13. Zimmermann G., Kelly G. J., Latzko E. Efficient purification and molecular properties of spinach chloroplast fructose 1,6-bisphosphatase. Eur J Biochem. 1976 Nov 15;70(2):361–367. doi: 10.1111/j.1432-1033.1976.tb11025.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES