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. 1980 Jan 15;186(1):169–176. doi: 10.1042/bj1860169

Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles.

B Hildmann, C Storelli, W Haase, M Barac-Nieto, H Murer
PMCID: PMC1161516  PMID: 7370006

Abstract

Uptake of L-lactate into rabbit jejunal brush-border-membrane vesicles prepared by a Ca2+-precipitation procedure was studied by a rapid filtration technique with L-[14C]-lactate as tracer. Transport of L-lactate into an intravesicular (osmotically reactive) space could be established. An inwardly directed NaCl gradient (outside 21 mM/inside 0mM) stimulated the uptake of L-lactate at 15 s 2-4-fold compared with that observed with an equal KCl gradient. A transient accumulation of L-lactate inside the vesicles (overshoot) was observed in the presence of an NaCl gradient. Gradients of LiCl, RbCl, CsCl or choline chloride were not able to replace NaCl in the stimulation of L-lactate uptake. L-Lactate uptake was saturable only in the presence of Na+. D-Lactate, DL-thiolactate (2-DL-mercaptopropionate), pyruvate and propionate inhibited the Na+-stimulated L-lactate uptake; D-lactate, thiolactate and pyruvate provoked trans-stimulation of L-lactate uptake. Artificially imposed diffusion potentials (inside negative) did not exert any effect on the Na+-dependent L-lactate uptake. The results are consistent with the existence of an electroneutral Na+/L-lactate co-transport system in the brush border of rabbit small intestine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berner W., Kinne R., Murer H. Phosphate transport into brush-border membrane vesicles isolated from rat small intestine. Biochem J. 1976 Dec 15;160(3):467–474. doi: 10.1042/bj1600467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berner W., Kinne R. Transport of p-aminohippuric acid by plasma membrane vesicles isolated from rat kidney cortex. Pflugers Arch. 1976 Feb 24;361(3):269–277. doi: 10.1007/BF00587292. [DOI] [PubMed] [Google Scholar]
  3. Evers C., Haase W., Murer H., Kinne R. Properties of brush border vesicles isolated from rat kidney cortex by calcium precipitation. Membr Biochem. 1978;1(3-4):203–219. doi: 10.3109/09687687809063848. [DOI] [PubMed] [Google Scholar]
  4. Hopfer U., Nelson K., Perrotto J., Isselbacher K. J. Glucose transport in isolated brush border membrane from rat small intestine. J Biol Chem. 1973 Jan 10;248(1):25–32. [PubMed] [Google Scholar]
  5. Hopfer U. Transport in isolated plasma membranes. Am J Physiol. 1978 Feb;234(2):F89–F96. doi: 10.1152/ajprenal.1978.234.2.F89. [DOI] [PubMed] [Google Scholar]
  6. Kessler M., Acuto O., Storelli C., Murer H., Müller M., Semenza G. A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline transport systems. Biochim Biophys Acta. 1978 Jan 4;506(1):136–154. doi: 10.1016/0005-2736(78)90440-6. [DOI] [PubMed] [Google Scholar]
  7. Lúcke H., Berner W., Menge H., Murer H. Sugar transport by brush border membrane vesicles isolated from human small intestine. Pflugers Arch. 1978 Mar 20;373(3):243–248. doi: 10.1007/BF00580831. [DOI] [PubMed] [Google Scholar]
  8. Lücke H., Stange G., Kinne R., Murer H. Taurocholate--sodium co-transport by brush-border membrane vesicles isolated from rat ileum. Biochem J. 1978 Sep 15;174(3):951–958. doi: 10.1042/bj1740951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murer H., Hopfer U. Demonstration of electrogenic Na+-dependent D-glucose transport in intestinal brush border membranes. Proc Natl Acad Sci U S A. 1974 Feb;71(2):484–488. doi: 10.1073/pnas.71.2.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Murer H., Hopfer U., Kinne-Saffran E., Kinne R. Glucose transport in isolated brush-border and lateral-basal plasma-membrane vesicles from intestinal epithelial cells. Biochim Biophys Acta. 1974 Apr 29;345(2):170–179. doi: 10.1016/0005-2736(74)90256-9. [DOI] [PubMed] [Google Scholar]
  11. Murer H., Hopfer U., Kinne R. Sodium/proton antiport in brush-border-membrane vesicles isolated from rat small intestine and kidney. Biochem J. 1976 Mar 15;154(3):597–604. [PMC free article] [PubMed] [Google Scholar]
  12. Pressman B. C. Ionophorous antibiotics as models for biological transport. Fed Proc. 1968 Nov-Dec;27(6):1283–1288. [PubMed] [Google Scholar]
  13. Pritchard P. J., Porteous J. W. Steady-state metabolism and transport of D-glucose by rat small intestine in vitro. Biochem J. 1977 Apr 15;164(1):1–14. doi: 10.1042/bj1640001a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schmitz J., Preiser H., Maestracci D., Ghosh B. K., Cerda J. J., Crane R. K. Purification of the human intestinal brush border membrane. Biochim Biophys Acta. 1973 Sep 27;323(1):98–112. doi: 10.1016/0005-2736(73)90434-3. [DOI] [PubMed] [Google Scholar]
  15. Sigrist-Nelson K., Murer H., Hopfer U. Active alanine transport in isolated brush border membranes. J Biol Chem. 1975 Jul 25;250(14):5674–5680. [PubMed] [Google Scholar]
  16. Sottocasa G. L., Kuylenstierna B., Ernster L., Bergstrand A. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol. 1967 Feb;32(2):415–438. doi: 10.1083/jcb.32.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]

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