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. 1980 Feb 15;186(2):439–441. doi: 10.1042/bj1860439

Seed extracts inhibiting protein synthesis in vitro.

A Gasperi-Campani, L Barbieri, P Morelli, F Stirpe
PMCID: PMC1161594  PMID: 7378060

Abstract

Of 33 seed extracts examined, 12 inhibited protein synthesis in a rabbit reticulocyte lysate. This activity seems to be due to a protein, since (i) it was recovered with the (NH4)2SO4 precipitate, (ii) it was retained by dialysis membranes, and (iii) in all cases but one was destroyed by boiling. Only the extracts from the seeds of Adenia digitata and, to a lower extent, of Euonymus europaeus inhibited protein synthesis in intact cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN E. H., SCHWEET R. S. Synthesis of hemoglobin in a cell-free system. I. Properties of the complete system. J Biol Chem. 1962 Mar;237:760–767. [PubMed] [Google Scholar]
  2. Barbieri L., Lorenzoni E., Stirpe F. Inhibition of protein synthesis in vitro by a lectin from Momordica charantia and by other haemagglutinins. Biochem J. 1979 Aug 15;182(2):633–635. doi: 10.1042/bj1820633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barbieri L., Zamboni M., Lorenzoni E., Montanaro L., Sperti S., Stirpe F. Inhibition of protein synthesis in vitro by proteins from the seeds of Momordica charantia (bitter pear melon). Biochem J. 1980 Feb 15;186(2):443–452. doi: 10.1042/bj1860443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ersson B., Aspberg K., Porath J. The phytohemagglutinin from sunn hemp seeds (Crotalaria juncea). Purification by biospecific affinity chromatography. Biochim Biophys Acta. 1973 Jun 15;310(2):446–452. doi: 10.1016/0005-2795(73)90128-1. [DOI] [PubMed] [Google Scholar]
  5. Gasperi-Campani A., Barbieri L., Lorenzoni E., Montanaro L., Sperti S., Bonetti E., Stirpe F. Modeccin, the toxin of Adenia digitata. Purification, toxicity and inhibition of protein synthesis in vitro. Biochem J. 1978 Aug 15;174(2):491–496. doi: 10.1042/bj1740491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gasperi-Campani A., TBarbieri L., Lorenzoni E., Stirpe F. Inhibition of protein synthesis by seed-extracts. A screening study. FEBS Lett. 1977 Apr 15;76(2):173–176. doi: 10.1016/0014-5793(77)80145-2. [DOI] [PubMed] [Google Scholar]
  7. Irvin J. D. Purification and partial characterization of the antiviral protein from Phytolacca americana which inhibits eukaryotic protein synthesis. Arch Biochem Biophys. 1975 Aug;169(2):522–528. doi: 10.1016/0003-9861(75)90195-2. [DOI] [PubMed] [Google Scholar]
  8. Obrig T. G., Irvin J. D., Hardesty B. The effect of an antiviral peptide on the ribosomal reactions of the peptide elongation enzymes, EF-I and EF-II. Arch Biochem Biophys. 1973 Apr;155(2):278–289. doi: 10.1016/0003-9861(73)90116-1. [DOI] [PubMed] [Google Scholar]
  9. Olsnes S., Haylett T., Refsnes K. Purification and characterization of the highly toxic lectin modeccin. J Biol Chem. 1978 Jul 25;253(14):5069–5073. [PubMed] [Google Scholar]
  10. PUCK T. T., CIECIURA S. J., FISHER H. W. Clonal growth in vitro of human cells with fibroblastic morphology; comparison of growth and genetic characteristics of single epithelioid and fibroblast-like cells from a variety of human organs. J Exp Med. 1957 Jul 1;106(1):145–158. doi: 10.1084/jem.106.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Refsnes K., Haylett T., Sandvig K., Olsnes S. Modeccin--a plant toxin inhibiting protein synthesis. Biochem Biophys Res Commun. 1977 Dec 21;79(4):1176–1183. doi: 10.1016/0006-291x(77)91130-5. [DOI] [PubMed] [Google Scholar]
  12. Sperti S., Montanaro L., Derenzini M., Gasperi-Campani A., Stirpe F. Effect of modeccin on rat liver ribosomes in vivo. Biochim Biophys Acta. 1979 May 24;562(3):495–503. doi: 10.1016/0005-2787(79)90112-6. [DOI] [PubMed] [Google Scholar]
  13. Sperti S., Montanaro L., Mattioli A., Testoni G., Stirpe F. Inhibition of protein synthesis in vitro by crotins and ricin. Effect on the steps of peptide chain elongation. Biochem J. 1976 Apr 15;156(1):7–13. doi: 10.1042/bj1560007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stewart T. S., Hruby D. E., Sharma O. K., Roberts W. K. An ATP-dependent inhibition of protein synthesis in ascites cell extracts by wheat germ protein. Biochim Biophys Acta. 1977 Nov 2;479(1):31–38. doi: 10.1016/0005-2787(77)90123-x. [DOI] [PubMed] [Google Scholar]
  15. Stirpe F., Gasperi-Campani A., Barbieri L., Lorenzoni E., Montanaro L., Sperti S., Bonetti E. Inhibition of protein synthesis by modeccin, the toxin of Modecca digitata. FEBS Lett. 1977 Dec 20;85(1):65–67. doi: 10.1016/0014-5793(78)81249-6. [DOI] [PubMed] [Google Scholar]
  16. Stirpe F., Pession-Brizzi A., Lorenzoni E., Strocchi P., Montanaro L., Sperti S. Studies on the proteins from the seeds of Croton tiglium and of Jatropha curcas. Toxic properties and inhibition of protein synthesis in vitro. Biochem J. 1976 Apr 15;156(1):1–6. doi: 10.1042/bj1560001. [DOI] [PMC free article] [PubMed] [Google Scholar]

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