Table 1. List of antibodies used in Western blots.
Each antibody is listed by the name used in the text. The source of the antibody, solvent, dilution ratio, and host species are also provide.CST, Cell Signaling Technology. TBS, tris-buffered saline; BSA, bovine serum albumin.
| Antibody | Catalog # | Solvent | Dilution | Host species |
|---|---|---|---|---|
| Total EGFR | 4267S, CST | 5% milk in TBS | 1:1000 | Rabbit |
| pEGFR(Y1068) | 3777S, CST | 3% BSA in TBS | 1:1000 | Rabbit |
| Total EphA2 | 97535S, CST | 5% Milk | 1:1000 | Rabbit |
| pEphA2(Y588) | 12677, CST | 3% BSA | 1:1000 | Rabbit |
| pEphA2(S897) | 6347S, CST | 3% BSA | 1:1000 | Rabbit |
| Actin | 3700S, CST | 5% milk | 1:5000 | Mouse |
| Anti-mouse | 925-68020, LiCore | 5% milk | 1:5000 | Goat |
| Anti-rabbit | 926-32211, LiCore | 5% milk | 1:5000 | Goat |