Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1980 Mar 15;186(3):971–975. doi: 10.1042/bj1860971

Effect of tunicamycin and cycloheximide on the secretion of acid hydrolases from I-cell cultured fibroblasts.

A L Miller, B C Kress, L Lewis, R Stein, C Kinnon
PMCID: PMC1161736  PMID: 7396846

Abstract

I-cell cultures fibroblasts secrete excessive amounts of N-acetyl-beta-D-hexosaminidase and alpha-L-fucosidase into the culture media as compared with normal fibroblasts. Addition of tunicamycin or cyd [14C]leucine (40--50%) into trichloroacetic acid-precipitable material decreased the secretion of these I-cell hydrolases to normal values within 24 h, but had no effect on the secretion of acid hydrolases from normal fibroblasts. These results indicate that I-cell cultured fibroblasts secrete at least two types of acid hydrolases: one is tunicamycin- and cycloheximide-sensitive and constitutes the greater proportion of the secreted hydrolases, and a smaller proportion is insensitive to tunicamycin and cycloheximide, similar t9 the acid hydrolases secreted by normal cultured fibroblasts.

Full text

PDF
971

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alhadeff J. A., Miller A. L., Wenger D. A., O'Brien J. S. Electrophoretic forms of human liver alpha-L-fucosidase and their relationship to fucosidosis (mucopolysaccharidosis F). Clin Chim Acta. 1974 Dec 17;57(3):307–313. doi: 10.1016/0009-8981(74)90412-4. [DOI] [PubMed] [Google Scholar]
  2. Amenta J. S., Sargus M. J., Baccino F. M. Control of cell protein degradation. Changes in activities of lysosomal proteases. Biochim Biophys Acta. 1977 Jun 3;476(3):253–261. doi: 10.1016/0005-2787(77)90008-9. [DOI] [PubMed] [Google Scholar]
  3. Amenta J. S., Sargus M. J., Baccino F. M. Inhibition of basal protein degradation in rat embryo fibroblasts by cycloheximide: correlation with activities of lysosomal proteases. J Cell Physiol. 1978 Dec;97(3 Pt 1):267–283. doi: 10.1002/jcp.1040970302. [DOI] [PubMed] [Google Scholar]
  4. De Duve C., Wattiaux R. Functions of lysosomes. Annu Rev Physiol. 1966;28:435–492. doi: 10.1146/annurev.ph.28.030166.002251. [DOI] [PubMed] [Google Scholar]
  5. Hickman S., Neufeld E. F. A hypothesis for I-cell disease: defective hydrolases that do not enter lysosomes. Biochem Biophys Res Commun. 1972 Nov 15;49(4):992–999. doi: 10.1016/0006-291x(72)90310-5. [DOI] [PubMed] [Google Scholar]
  6. Hickman S., Shapiro L. J., Neufeld E. F. A recognition marker required for uptake of a lysosomal enzyme by cultured fibroblasts. Biochem Biophys Res Commun. 1974 Mar 15;57(1):55–61. doi: 10.1016/s0006-291x(74)80356-6. [DOI] [PubMed] [Google Scholar]
  7. Kress B. C., Miller A. L. Urinary lysosomal hydrolases in mucolipidosis II and mucolipidosis III. Biochem J. 1979 Feb 1;177(2):409–415. doi: 10.1042/bj1770409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Leroy J. G., Ho M. W., MacBrinn M. C., Zielke K., Jacob J., O'Brien J. S. I-cell disease: biochemical studies. Pediatr Res. 1972 Oct;6(10):752–757. doi: 10.1203/00006450-197210000-00002. [DOI] [PubMed] [Google Scholar]
  10. Lloyd J. B. Cellular transport of lysosomal enzymes: an alternative hypothesis. Biochem J. 1977 Apr 15;164(1):281–282. doi: 10.1042/bj1640281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Takatsuki A., Tamura G. Effect of tunicamycin on the synthesis of macromolecules in cultures of chick embryo fibroblasts infected with Newcastle disease virus. J Antibiot (Tokyo) 1971 Nov;24(11):785–794. doi: 10.7164/antibiotics.24.785. [DOI] [PubMed] [Google Scholar]
  12. Wiesmann U. N., Herschkowitz N. N. Studies on the pathogenetic mechanism of I-cell disease in cultured fibroblasts. Pediatr Res. 1974 Nov;8(11):865–869. doi: 10.1203/00006450-197411000-00002. [DOI] [PubMed] [Google Scholar]
  13. Zagury D., Uhr J. W., Jamieson J. D., Palade G. E. Immunoglobulin synthesis and secretion. II. Radioautographic studies of sites of addition of carbohydrate moieties and intracellular transport. J Cell Biol. 1970 Jul;46(1):52–63. doi: 10.1083/jcb.46.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. von Figura K., Weber E. An alternative hypothesis of cellular transport of lysosomal enzymes in fibroblasts. Effect of inhibitors of lysosomal enzyme endocytosis on intra- and extra-cellular lysosomal enzyme activities. Biochem J. 1978 Dec 15;176(3):943–950. doi: 10.1042/bj1760943. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES