Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1980 Mar 15;186(3):987–992. doi: 10.1042/bj1860987

Role of Ca2+ and cyclic nucleotides in the control of prostaglandin E production in the rat anterior pituitary gland.

A Betteridge
PMCID: PMC1161738  PMID: 6156672

Abstract

In an attempt to relate changes in the intracellular concentration of prostaglandin E to the secretion process, two agents known to increase cyclic nucleotide concentrations and hormone release were added to dispersed rat anterior pituitary cells. They caused increases in teh intracellular prostaglandin E concentrations. Increasing the K+ concentration in the medium (which stimulates hormone release) caused a rapid rise in prostaglandin E concentrations. The addition of the Ca2'onophore A23187 had a similar effect. The effects of changes in the K+ and Ca2+concentrations and the addition of EDTA were measured on the redistribution of radioactivity in pituitary glands prelabelled with [3H]arachidonic acid. Elevated K+ concentrations stimulated the transfer of label to prostaglandins and free arachidonic acid, suggesting an increased phospholipase A activity. On the other hand, the absence of extracellular CaCl2 and the addition of EDTA had the opposite effect, which could be cancelled by the addition of sufficient amounts. of CaCl2. It is concluded that the addition of agents that increase membrane permeability to bivalent cations probably results in an influx of Ca2+ and this appears to result in increased phospholipase A activity, which in turn leads to an increase in prostaglandin production.

Full text

PDF
987

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashby J. P., Speake R. N. Insulin and glucagon secretion from isolated islets of Langerhans. The effects of calcium ionophores. Biochem J. 1975 Jul;150(1):89–96. [PMC free article] [PubMed] [Google Scholar]
  2. Barden N., Betteridge A. Stimulation of prostaglandin accumulation in the rat anterior pituitary gland by luteinizing hormone releasing hormone in vitro. J Endocrinol. 1977 Nov;75(2):277–283. doi: 10.1677/joe.0.0750277. [DOI] [PubMed] [Google Scholar]
  3. Betteridge A., Wallis M. Biosynthesis of growth hormone in the rat anterior pituitary gland. Stimulation of biosynthesis in vitro by insulin. Biochem J. 1973 Aug;134(4):1103–1113. doi: 10.1042/bj1341103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Betteridge A., Wallis M. Involvement of prostaglandins in the inhibition of growth hormone production in cultured pituitary cells by insulin. J Endocrinol. 1979 Feb;80(2):239–248. doi: 10.1677/joe.0.0800239. [DOI] [PubMed] [Google Scholar]
  5. Betteridge A., Wallis M. Role of prostaglandins and adenosine 3':5'-cyclic monophosphate in the control by insulin of growth-hormone synthesis in vitro. Biochem Soc Trans. 1977;5(1):224–226. doi: 10.1042/bst0050224. [DOI] [PubMed] [Google Scholar]
  6. Betteridge A., Wallis M. Stimulation of anterior pituitary prostaglandin E content and somatotropin (growth hormone) synthesis by phospholipase A. Biochem J. 1978 Oct 15;176(1):319–323. doi: 10.1042/bj1760319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bicknell R. J., Schofield J. G. Mechanism of action of somatostatin: inhibition of ionophore A23187-induced release of growth hormone from dispersed bovine pituitary cells. FEBS Lett. 1976 Sep 15;68(1):23–26. doi: 10.1016/0014-5793(76)80395-x. [DOI] [PubMed] [Google Scholar]
  8. DOUGLAS W. W., POISNER A. M. STIMULUS-SECRETION COUPLING IN A NEUROSECRETORY ORGAN: THE ROLE OF CALCIUM IN THE RELEASE OF VASOPRESSIN FROM THE NEUROHYPOPHYSIS. J Physiol. 1964 Jul;172:1–18. doi: 10.1113/jphysiol.1964.sp007399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Desbuquois B., Aurbach G. D. Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays. J Clin Endocrinol Metab. 1971 Nov;33(5):732–738. doi: 10.1210/jcem-33-5-732. [DOI] [PubMed] [Google Scholar]
  10. Foreman J. C., Mongar J. L., Gomperts B. D. Calcium ionophores and movement of calcium ions following the physiological stimulus to a secretory process. Nature. 1973 Oct 5;245(5423):249–251. doi: 10.1038/245249a0. [DOI] [PubMed] [Google Scholar]
  11. Gautvik K. M., Tashjian A. H., Jr Effects of cations and colchicine on the release of prolactin and growth hormone by functional pituitary tumor cells in culture. Endocrinology. 1973 Oct;93(4):793–799. doi: 10.1210/endo-93-4-793. [DOI] [PubMed] [Google Scholar]
  12. Hertlelendy F., Todd H., Peake G. T., Machlin L. J., Johnston G., Pounds G. Studies on growth hormone secretion. I. Effects of dibutyryl cyclic AMP, theophylline, epinephrine, ammonium ion, and hypothalamic extracts on the release of growth hormone from rat anterior pituitaries in vitro. Endocrinology. 1971 Nov;89(5):1256–1262. doi: 10.1210/endo-89-5-1256. [DOI] [PubMed] [Google Scholar]
  13. LaBella F., Dular R., Vivian S., Queen G. Pituitary hormone releasing or inhibiting activity of metal ions present in hypothalamic extracts. Biochem Biophys Res Commun. 1973 Jun 8;52(3):786–791. doi: 10.1016/0006-291x(73)91006-1. [DOI] [PubMed] [Google Scholar]
  14. Lemay A., Labrie F. Calcium-dependent stimulation of prolactin release in rat anterior pituitary in vitro by n(6)-monobutyryl adenosine 3',5'-monophosphate. FEBS Lett. 1972 Jan 15;20(1):7–10. doi: 10.1016/0014-5793(72)80003-6. [DOI] [PubMed] [Google Scholar]
  15. McCann S. M., Porter J. C. Hypothalamic pituitary stimulating and inhibiting hormones. Physiol Rev. 1969 Apr;49(2):240–284. doi: 10.1152/physrev.1969.49.2.240. [DOI] [PubMed] [Google Scholar]
  16. Rubin R. P. The role of calcium in the release of neurotransmitter substances and hormones. Pharmacol Rev. 1970 Sep;22(3):389–428. [PubMed] [Google Scholar]
  17. Scarpa A., Inesi G. Ionophore mediated equilibration of calcium ion gradients in fragmented-sarcoplasmic reticulum. FEBS Lett. 1972 May 15;22(3):273–276. doi: 10.1016/0014-5793(72)80248-5. [DOI] [PubMed] [Google Scholar]
  18. Schofield J. G. Role of cyclic 3',5'-adenosine monophosphate in the release of growth hormone in vitro. Nature. 1967 Sep 23;215(5108):1382–1383. doi: 10.1038/2151382b0. [DOI] [PubMed] [Google Scholar]
  19. Vale W., Grant G., Amoss M., Blackwell R., Guillemin R. Culture of enzymatically dispersed pituitary cells: functional validation of a method. Endocrinology. 1972 Aug;91(2):562–572. doi: 10.1210/endo-91-2-562. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES