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. 1979 Nov 15;184(2):221–227. doi: 10.1042/bj1840221

Isolation of material displaying insulin-like immunological biological activity from the brain of the blowfly Calliphora vomitoria.

H Duve, A Thorpe, N R Lazarus
PMCID: PMC1161755  PMID: 534526

Abstract

An insulin-like material from the brain of the blowfly Calliphora vomitoria was partially purified by acid alcohol extraction, gel filtration and ion-exchange cellulose chromatography. In addition, the RF value on polyacrylamide-gel electrophoresis was determined. The material was characterized by its ability to cross-react with bovine insulin antibody and by displaying diminished immunoreactivity on dilution. It displaced specifically bound 125I-labelled insulin from rat liver plasma membrane insulin receptors and displayed insulin-like biological activity on the isolated rat fat-cell. Within 30 min of injection into Calliphora, made hypertrehalocaemic and hyperglucaemic as a result of median neurosecretory cell removal, it caused the concentrations of both sugars to return to normal. The hypothesis is put forward that the median neurosecretory cells are the source of the material.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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