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. 1980 May 1;187(2):525–528. doi: 10.1042/bj1870525

Properties of a glycopeptide isolated from human Tamm-Horsfall glycoprotein. Interaction with leucoagglutinin and anti-(human Tamm-Horsfall glycoprotein) antibodies.

A Abbondanza, C Franceschi, F Licastro, F Serafini-Cessi
PMCID: PMC1161820  PMID: 6967312

Abstract

A sialylated glycopeptide isolated after Pronase digestion of human Tamm-Horsfall glycoprotein behaves as a powerful monovalent hapten in the precipitin reaction between human Tamm-Horsfall glycoprotein and leucoagglutinin, but fails to inhibit the interaction of the glycoprotein with rabbit anti-(human Tamm-Horsfall glycoprotein) antibodies. The glycopeptide is much less active than the intact glycoprotein as an inhibitor of lymphocyte transformation induced by leucoagglutinin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Afonso A. M., Marshall R. D. Observations on the structure of the carbohydrate moieties of the Tamm-Horsfall glycoprotein [proceedings]. Biochem Soc Trans. 1979 Feb;7(1):170–173. doi: 10.1042/bst0070170. [DOI] [PubMed] [Google Scholar]
  2. Allen A. K., Desai N. N., Neuberger A., Creeth J. M. Properties of potato lectin and the nature of its glycoprotein linkages. Biochem J. 1978 Jun 1;171(3):665–674. doi: 10.1042/bj1710665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bloomfield F. J., Dunstan D. R., Foster C. L., Serafini-Cessi F., Marshall R. D. Some factors affecting the production, by cultured baby-hamster kidney cells, of BHK glycoprotein I which cross-reacts immunologically with Tamm-Horsfall glycoprotein. Biochem J. 1977 Apr 15;164(1):41–51. doi: 10.1042/bj1640041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CESSI C., PILIEGO F. The determination of amino sugars in the presence of amino acids and glucose. Biochem J. 1960 Dec;77:508–510. doi: 10.1042/bj0770508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CESSI C., SERAFINI-CESSI F. A method for the determination of D-galactosamine in the presence of D-glucosamine. Biochem J. 1963 Jul;88:132–136. doi: 10.1042/bj0880132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FRANCOIS C., MARSHALL R. D., NEUBERGER A. Carbohydrates in protein. 4. The determination of mannose in hen's-egg albumin by radioisotope dilution. Biochem J. 1962 May;83:335–341. doi: 10.1042/bj0830335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fletcher A. P., Neuberger A., Ratcliffe W. A. Tamm-Horsfall urinary glycoprotein. The chemical composition. Biochem J. 1970 Nov;120(2):417–424. doi: 10.1042/bj1200417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fletcher A. P., Neuberger A., Ratcliffe W. A. Tamm-Horsfall urinary glycoprotein. The subunit structure. Biochem J. 1970 Nov;120(2):425–432. doi: 10.1042/bj1200425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grant A. M., Neuberger A. The development of a radioimmunoassay for the measurement of urinary Tamm-Horsfall glycoprotein in the presence of sodium dodecyl sulphate. Clin Sci. 1973 Feb;44(2):163–179. doi: 10.1042/cs0440163. [DOI] [PubMed] [Google Scholar]
  10. Hellström U., Dillner M. L., Hammarström S., Perlmann P. The interaction of nonmitogenic and mitogenic lectins with T lymphocytes: association of cellular receptor sites. Scand J Immunol. 1976;5(1-2):45–54. [PubMed] [Google Scholar]
  11. MOORE S., STEIN W. H. A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J Biol Chem. 1954 Dec;211(2):907–913. [PubMed] [Google Scholar]
  12. Marchesi V. T., Furthmayr H., Tomita M. The red cell membrane. Annu Rev Biochem. 1976;45:667–698. doi: 10.1146/annurev.bi.45.070176.003315. [DOI] [PubMed] [Google Scholar]
  13. Means G. E., Feeney R. E. Reductive alkylation of amino groups in proteins. Biochemistry. 1968 Jun;7(6):2192–2201. doi: 10.1021/bi00846a023. [DOI] [PubMed] [Google Scholar]
  14. Serafini-Cessi F., Franceschi C., Sperti S. Specific interaction of human Tamm-Horsfall gylcoprotein with leucoagglutinin, a lectin from Phaseolus vulgaris (red kidney bean). Biochem J. 1979 Nov 1;183(2):381–388. doi: 10.1042/bj1830381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sikri K. L., Foster C. L., Bloomfield F. J., Marshall R. D. Localization by immunofluorescence and by light- and electron-microscopic immunoperoxidase techniques of Tamm-Horsfall glycoprotein in adult hamster kidney. Biochem J. 1979 Sep 1;181(3):525–532. doi: 10.1042/bj1810525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. TAMM I., HORSFALL F. L., Jr A mucoprotein derived from human urine which reacts with influenza, mumps, and Newcastle disease viruses. J Exp Med. 1952 Jan;95(1):71–97. doi: 10.1084/jem.95.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]

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