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. 1980 May 15;188(2):337–343. doi: 10.1042/bj1880337

Specific inactivation of lysosomal glycosidases in living fibroblasts by the corresponding glycosylmethyl-p-nitrophenyltriazenes.

O P Van Diggelen, H Galjaard, M L Sinnott, P J Smith
PMCID: PMC1161875  PMID: 6772162

Abstract

1. beta-D-Galactopyranosylmethyl-p-nitrophenyltriazene can specificag detectable changes in cell viability or the activities of other hydrolases. 2. beta-D-Glucopyranosylmethyl-p-nitrophenyltriazene behaves similarly towards lysosomal beta-glucosidase, but also inactivates some alpha-glucosidase. 3. Both beta-galactosidase and beta-glucosidase activities in triazene-treated confluent fibroblast cultures recover exponentially; if zero-order enzyme production is assumed, turnover times of 10 and 5 days respectively can be estimated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Yoseph Y., Fiddler M. B., Rousson R., Nadler H. L. Multiple carbohydrate-cleaving specificities in human acidic and neutral glycosidases. Biochim Biophys Acta. 1979 Jun 6;568(2):386–394. doi: 10.1016/0005-2744(79)90307-3. [DOI] [PubMed] [Google Scholar]
  2. Chen T. R. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res. 1977 Feb;104(2):255–262. doi: 10.1016/0014-4827(77)90089-1. [DOI] [PubMed] [Google Scholar]
  3. Chiao Y. B., Peters S. P., Diven W. F., Lee R. E., Glew R. H. Demonstration of a deficiency of beta-xylosidase activity in various forms of Gaucher's disease. Metabolism. 1979 Jan;28(1):56–62. doi: 10.1016/0026-0495(79)90168-9. [DOI] [PubMed] [Google Scholar]
  4. Gatt S., Rapport M. M. Isolation of beta-galactosidase and beta-glucosidase from brain. Biochim Biophys Acta. 1966 Mar 7;113(3):567–576. doi: 10.1016/s0926-6593(66)80014-0. [DOI] [PubMed] [Google Scholar]
  5. Kleijer W. J., Hoogeveen A., Verheijen F. W., Niermeijer M. F., Galjaard H., O'Brien J. S., Warner T. G. Prenatal diagnosis of sialidosis with combined neuraminidase and beta-galactosidase deficiency. Clin Genet. 1979 Jul;16(1):60–61. doi: 10.1111/j.1399-0004.1979.tb00851.x. [DOI] [PubMed] [Google Scholar]
  6. Lowden J. A., O'Brien J. S. Sialidosis: a review of human neuraminidase deficiency. Am J Hum Genet. 1979 Jan;31(1):1–18. [PMC free article] [PubMed] [Google Scholar]
  7. Norden A. G., Tennant L. L., O'Brien J. S. GM1 ganglioside beta-galactosidase. A. Purification and studies of the enzyme from human liver. J Biol Chem. 1974 Dec 25;249(24):7969–7976. [PubMed] [Google Scholar]
  8. Ockerman P. A. Identity of beta-glucosidase, beta-xylosidase and one of the beta-galactosidase activities in human liver when assayed with 4-methylumbelliferyl-beta-D-glycosides studies in cases of Gaucher's disease. Biochim Biophys Acta. 1968 Aug 6;165(1):59–62. [PubMed] [Google Scholar]
  9. PRICE V. E., STERLING W. R., TARANTOLA V. A., HARTLEY R. W., Jr, RECHCIGL M., Jr The kinetics of catalase synthesis and destruction in vivo. J Biol Chem. 1962 Nov;237:3468–3475. [PubMed] [Google Scholar]
  10. Sinnott M. L., Smith P. J. Affinity labelling with a deaminatively generated carbonium ion. Kinetics and stoicheiometry of the alkylation of methionine-500 of the lacZ beta-galactosidase of Escherichia coli by beta-D-galactopyranosylmethyl-p-nitrophenyltriazene. Biochem J. 1978 Nov 1;175(2):525–538. doi: 10.1042/bj1750525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Warburton M. J., Wynn C. H. The effect of intralysosomal sucrose storage on the turnover of hamster fibroblast lysosomal and Golgi-apparatus enzymes. Biochem J. 1976 Aug 15;158(2):401–407. doi: 10.1042/bj1580401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Warburton M. J., Wynn C. H. The turnover of hamster fibroblast lysosomal beta-D-glucuronidase. Biochem J. 1977 Jan 15;162(1):201–203. doi: 10.1042/bj1620201. [DOI] [PMC free article] [PubMed] [Google Scholar]

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