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. 1980 May 15;188(2):351–361. doi: 10.1042/bj1880351

Photosynthetic electron transport in a cell-free preparation from the thermophilic blue-green alga Phormidium laminosum.

A C Stewart, D S Bendall
PMCID: PMC1161877  PMID: 6772163

Abstract

1. A cell-free preparation of membrane fragments was prepared from the thermophilic blue-green alga Phormidium laminosum by lysozyme treatment of the cells followed by osmotic shock to lyse the spheroplasts. The membrane fragments showed high rates of photosynthetic electron transport and O2 evolution (180-250 mumol of O2/h per mg of chlorophyll a with 2,6-dimethyl-1,4-benzoquinone as electron acceptor). O2-evolution activity was stable provided that cations (e.g. 10mM-Mg2+ or 100mM-Na+) or glycerol (25%, v/v) were present in the suspending medium. 2. The components of the electron-transport chain in P. laminosum were similar to those of other blue-green algae: the cells contained Pigment P700, plastocyanin, soluble high-potential cytochrome c-553, soluble low-potential cytochrome c-54 and membrane-bound cytochromes f, b-563 and b-559 (both low- and high-potential forms). The amounts and midpoint potentials of the membrane-bound cytochromes were similar to those in higher-plant chloroplasts. 3. Although O2 evolution in P. laminosum spheroplasts was resistant to high temperatures, thermal stability was not retained in the cell-free preparation. However, in contrast with higher plants, O2 evolution in P. laminosum membrane fragments was remarkably resistant to the non-ionic detergent Triton X-100.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aitken A. Purification and primary structure of cytochrome f from the cyanobacterium, Plectonema boryanum. Eur J Biochem. 1977 Aug 15;78(1):273–279. doi: 10.1111/j.1432-1033.1977.tb11738.x. [DOI] [PubMed] [Google Scholar]
  2. Ambler R. P., Bartsch R. G. Amino acid sequence similarity between cytochrome f from a blue-green bacterium and algal chloroplasts. Nature. 1975 Jan 24;253(5489):285–288. doi: 10.1038/253285a0. [DOI] [PubMed] [Google Scholar]
  3. Aparicio P. J., Ando K., Arnon D. I. Photochemical activity and components of membrane preparations from blue-green algae. II. Low-temperature photooxidation of cytochrome b559. Biochim Biophys Acta. 1974 Aug 23;357(2):246–251. doi: 10.1016/0005-2728(74)90064-4. [DOI] [PubMed] [Google Scholar]
  4. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Arnon D. I., McSwain B. D., Tsujimoto H. Y., Wada K. Photochemical activity and components of membrane preparations from blue-green algae. I. Coexistence of two photosystems in relation to chlorophyll a and removal of phycocyanin. Biochim Biophys Acta. 1974 Aug 23;357(2):231–245. doi: 10.1016/0005-2728(74)90063-2. [DOI] [PubMed] [Google Scholar]
  6. Biggins J. Photosynthetic Reactions by Lysed Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum. Plant Physiol. 1967 Oct;42(10):1447–1456. doi: 10.1104/pp.42.10.1447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Biggins J. Preparation of Metabolically Active Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum. Plant Physiol. 1967 Oct;42(10):1442–1446. doi: 10.1104/pp.42.10.1442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Binder A., Tel-or E., Avron M. Photosynthetic activities of membrane preparations of the blue-green alga Phormidium luridum. Eur J Biochem. 1976 Aug 1;67(1):187–196. doi: 10.1111/j.1432-1033.1976.tb10648.x. [DOI] [PubMed] [Google Scholar]
  9. Cockburn W., Walker D. A., Baldry C. W. The isolation of spinach chloroplasts in pyrophosphate media. Plant Physiol. 1968 Sep;43(9):1415–1418. doi: 10.1104/pp.43.9.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FREDRICKS W. W., JAGENDORF A. T. A SOLUBLE COMPONENT OF THE HILL REACTION IN ANACYSTIS NIDULANS. Arch Biochem Biophys. 1964 Jan;104:39–49. doi: 10.1016/s0003-9861(64)80032-1. [DOI] [PubMed] [Google Scholar]
  11. Fujita Y., Myers J. Kinetic analysis of light-induced cytochrome c redox reactions in Anabaena lamellar fragments. Arch Biochem Biophys. 1967 Mar;119(1):8–15. doi: 10.1016/0003-9861(67)90421-3. [DOI] [PubMed] [Google Scholar]
  12. Holton R. W., Myers J. Water-soluble cytochromes from a blue-green alga. I. Extraction, purification, and spectral properties of cytochromes C (549, 552, and 554, Anacystis nidulans). Biochim Biophys Acta. 1967 Mar 8;131(2):362–374. doi: 10.1016/0005-2728(67)90150-8. [DOI] [PubMed] [Google Scholar]
  13. Honeycutt R. C., Krogmann D. W. Further studies on the oxygen-reducing system of Anabaena variabilis. Biochim Biophys Acta. 1972 Feb 28;256(2):467–476. doi: 10.1016/0005-2728(72)90075-8. [DOI] [PubMed] [Google Scholar]
  14. KATOH S., SUGA I., SHIRATORI I., TAKAMIYA A. Distribution of plastocyanin in plants, with special reference to its localization in chloroplasts. Arch Biochem Biophys. 1961 Jul;94:136–141. doi: 10.1016/0003-9861(61)90020-0. [DOI] [PubMed] [Google Scholar]
  15. Kimimura M., Kato S. Studies on electron transport associated with photosystem I. I. Functional site of plastocyanin: inhibitory effects of HgCl 2 on electron transport and plastocyanin in chloroplasts. Biochim Biophys Acta. 1972 Nov 17;283(2):279–292. doi: 10.1016/0005-2728(72)90244-7. [DOI] [PubMed] [Google Scholar]
  16. Knaff D. B. Light-induced oxidation-reduction reactions in a cell-free preparation from the blue-green alga Nostoc muscorum: the role of cytochrome f, cytochrome b558, C550, and P700 in noncyclic electron transport. Biochim Biophys Acta. 1973 Nov 22;325(2):284–296. doi: 10.1016/0005-2728(73)90104-7. [DOI] [PubMed] [Google Scholar]
  17. Lightbody J. J., Krogmann D. W. Isolation and properties of plastocyanin from Anabaena variabilis. Biochim Biophys Acta. 1967 May 9;131(3):508–515. doi: 10.1016/0005-2728(67)90010-2. [DOI] [PubMed] [Google Scholar]
  18. McSwain B. D., Tsujimoto H. Y., Arnon D. I. Effects of magnesium and chloride ions on light-induced electron transport in membrane fragments from a blue-green alga. Biochim Biophys Acta. 1976 Feb 16;423(2):313–322. doi: 10.1016/0005-2728(76)90188-2. [DOI] [PubMed] [Google Scholar]
  19. Ogawa T., Vernon L. P., Mollenhauer H. H. Properties and structure of fractions prepared from Anabaena variabilis by the action Triton X-100. Biochim Biophys Acta. 1969 Feb 25;172(2):216–229. doi: 10.1016/0005-2728(69)90065-6. [DOI] [PubMed] [Google Scholar]
  20. Ono T., Murata N. Photosynthetic electron transport and phosphorylation reactions in thylakoid membranes from the blue-green alga Anacystis nidulans. Biochim Biophys Acta. 1978 Jun 8;502(3):477–485. doi: 10.1016/0005-2728(78)90080-4. [DOI] [PubMed] [Google Scholar]
  21. SUSOR W. A., KROGMANN D. W. HILL ACTIVITY IN CELL-FREE PREPARATIONS OF A BLUE-GREEN ALGA. Biochim Biophys Acta. 1964 Jul 29;88:11–19. doi: 10.1016/0926-6577(64)90150-0. [DOI] [PubMed] [Google Scholar]
  22. Stanier R. Y., Kunisawa R., Mandel M., Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev. 1971 Jun;35(2):171–205. doi: 10.1128/br.35.2.171-205.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stewart A. C., Bendall D. S. Preparation of active fractions enriched in photosystem I and photosystem II from the thermophilic blue-green alga Phormidium laminosum. Biochem Soc Trans. 1978;6(5):1040–1041. doi: 10.1042/bst0061040. [DOI] [PubMed] [Google Scholar]
  24. Stewart A. C., Bendall D. S. Preparation of an active, oxygen-evolving photosystem 2 particle from a blue-green alga. FEBS Lett. 1979 Nov 15;107(2):308–312. doi: 10.1016/0014-5793(79)80396-8. [DOI] [PubMed] [Google Scholar]
  25. Vernon L. P., Shaw E. Photochemical Activities of Spinach Chloroplasts Following Treatment with the Detergent Triton X-100. Plant Physiol. 1965 Nov;40(6):1269–1277. doi: 10.1104/pp.40.6.1269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wood P. M., Bendall D. S. The kinetics and specificity of electron transfer from cytochromes and copper proteins to P700. Biochim Biophys Acta. 1975 Apr 14;387(1):115–128. doi: 10.1016/0005-2728(75)90056-0. [DOI] [PubMed] [Google Scholar]
  27. Wood P. M. The roles of c-type cytochromes in algal photosynthesis. Extraction from algae of a cytochrome similar to higher plant cytochrome f. Eur J Biochem. 1977 Feb;72(3):605–612. doi: 10.1111/j.1432-1033.1977.tb11283.x. [DOI] [PubMed] [Google Scholar]

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