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. 1980 Jul 1;189(1):89–93. doi: 10.1042/bj1890089

Complexing reverse transcriptase with polyspermine-ribonuclease.

A K Bandyopadhyay, D Wang, C C Levy
PMCID: PMC1161920  PMID: 6161606

Abstract

Polyspermine-ribonuclease (Mr approximately 17 000) and the enzyme transcriptase from Rauscher-leukaemia virus (Mr approximately 70 000) form a complex Mr approx. 160 000) such that the molar ratio of polyspermine-ribonuclease to reverse transcriptase is 5:1. The most favourable condition for complex-formation is in a solution consisting of 0.01 M-Tris/HCl buffer, pH 7.5, 0.25 M-KCl and 1 mM-Mn2+ at 37 degrees C. The association of the two enzymes retains full RNAase activity, but reverse-transcriptase activity is completely inhibited when ribonuclease-sensitive polymers such as (dG)12 x (rC)n or viral 70S RNA are used as primer templates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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