Abstract
A simple microassay for the determination of UDP-glucuronic acid was developed on the basis of the formation of benzo[a]pyrene 3-glucuronide catalysed by UDP-glucuronyltransferase of guinea-pig liver. As little as 1-5 pmol of UDP-glucuronic acid was detectable in extracts of heat-denatured probes of liver or cultured cells equivalent to 10-50 micrograms of cellular protein.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bock K. W., Huber E., Schlote W. UDP-glucuronyltransferase in perfused rat liver and in microsomes. Effects of CCl4 injury. Naunyn Schmiedebergs Arch Pharmacol. 1977 Feb;296(3):199–203. doi: 10.1007/BF00498686. [DOI] [PubMed] [Google Scholar]
- Lehnert W., Wagner T. O., Fürst M. W., Künzer W. Eine radioenzymatische Mikromethode zur Bestimmung der UDP-Glucuronsäure. J Clin Chem Clin Biochem. 1979 Jun;17(6):363–367. [PubMed] [Google Scholar]
- Singh J., Wiebel F. J. A highly sensitive and rapid fluorometric assay for UDP-glucuronyltransferase using 3-hydroxybenzo (a) pyrene as substrate. Anal Biochem. 1979 Oct 1;98(2):394–401. doi: 10.1016/0003-2697(79)90158-1. [DOI] [PubMed] [Google Scholar]
- Wiebel F. J., Leutz J. C., Diamond L., Gelboin H. V. Aryl hydrocarbon (benzo(a)pyrene) hydroxylase in microsomes from rat tissues: differential inhibition and stimulation by benzoflavones and organic solvents. Arch Biochem Biophys. 1971 May;144(1):78–86. doi: 10.1016/0003-9861(71)90456-5. [DOI] [PubMed] [Google Scholar]
- Wong K. P., Lau Y. K. Assay of UDPG1cUA pyrophosphatase and its relation to transglucuronidation. Biochim Biophys Acta. 1970 Oct 14;220(1):61–68. doi: 10.1016/0005-2744(70)90229-9. [DOI] [PubMed] [Google Scholar]
- Wong K. P. Measurement of nanogram quantities of UDP-glucuronic acid in tissues. Anal Biochem. 1977 Oct;82(2):559–563. doi: 10.1016/0003-2697(77)90194-4. [DOI] [PubMed] [Google Scholar]