Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1980 Aug 1;189(2):377–380. doi: 10.1042/bj1890377

The separation and purification of rat liver UDP-glucuronyltransferase activities towards testosterone and oestrone.

P J Weatherill, B Burchell
PMCID: PMC1162010  PMID: 6779813

Abstract

1. Reconstitution of UDP-glucuronyltransferase preparations with phosphataidylcholine liposomes facilitated the purification of testosterone UDP-glucuronyltransferase. 2. Transferase activity towards testosterone co-purifies with that towards 4-nitrophenol. 3. UDP-glucuronyltransferase activity towards oestrone was separated from that towards testosterone. 4. These results suggest that testosterone and 4-nitrophenol may be glucuronidated by a different form of UDP-glucuronyltransferase from the one glucuronidating oestrone.

Full text

PDF
377

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bock K. W., Josting D., Lilienblum W., Pfeil H. Purification of rat-liver microsomal UDP-glucuronyltransferase. Separation of two enzyme forms inducible by 3-methylcholanthrene or phenobarbital. Eur J Biochem. 1979 Jul;98(1):19–26. doi: 10.1111/j.1432-1033.1979.tb13155.x. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Burchell B., Hallinan T. Phospholipid content and activity of pure uridine diphosphate-glucuronyltransferase from rat liver. Biochem J. 1978 Jun 1;171(3):821–824. doi: 10.1042/bj1710821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burchell B. Isolation and purification of bilirubin UDP-glucuronyl-transferase from rat liver. FEBS Lett. 1980 Feb 25;111(1):131–135. doi: 10.1016/0014-5793(80)80777-0. [DOI] [PubMed] [Google Scholar]
  5. Burchell B. Purification of UDP-glucuronyltransferase from untreated rat liver. FEBS Lett. 1977;78(1):101–104. doi: 10.1016/0014-5793(77)80283-4. [DOI] [PubMed] [Google Scholar]
  6. Burchell B. Substrate specificity and properties of uridine diphosphate glucuronyltransferase purified to apparent homogeneity from phenobarbital-treated rat liver. Biochem J. 1978 Sep 1;173(3):749–757. doi: 10.1042/bj1730749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gorski J. P., Kasper C. B. Purification and properties of microsomal UDP-glucuronosyltransferase from rat liver. J Biol Chem. 1977 Feb 25;252(4):1336–1343. [PubMed] [Google Scholar]
  8. Jansen P. L., Arias I. M. Delipidation and reactivation of UDPglucuronosyltransferase from rat liver. Biochim Biophys Acta. 1975 May 23;391(1):23–38. [PubMed] [Google Scholar]
  9. Rao G. S., Breuer H. Partial purification and kinetic properties of a soluble estrogen glucuronyltransferase from pig intestine. J Biol Chem. 1969 Oct 25;244(20):5521–5527. [PubMed] [Google Scholar]
  10. Rao G. S., Haueter G., Rao M. L., Breuer H. An improved assay for steroid glucuronyltransferase in rat liver microsomes. Anal Biochem. 1976 Jul;74(1):35–40. doi: 10.1016/0003-2697(76)90307-9. [DOI] [PubMed] [Google Scholar]
  11. Rao G. S., Rao M. L., Breuer H. Partial purification and kinetics of oestriol 16 alpha-glucuronyltransferase from the cytosol fraction of human liver. Biochem J. 1970 Jul;118(4):625–634. doi: 10.1042/bj1180625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rao G. S., Rao M. L., Breuer H. Studies on a testosterone glucuronyltransferase from the cytosol fraction of human liver. Biochem J. 1970 Oct;119(4):635–642. doi: 10.1042/bj1190635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tukey R. H., Billings R. E., Autor A. P., Tephly T. R. Phospholipid-dependence of oestrone UDP-glucuronyltransferase and p-nitrophenol UDP-glucuronyltransferase. Biochem J. 1979 Apr 1;179(1):59–65. doi: 10.1042/bj1790059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tukey R. H., Billings R. E., Tephly T. R. Separation of oestrone UDP-glucuronyltransferase and p-nitrophenol UDP-glucuronyltransferase activities. Biochem J. 1978 Jun 1;171(3):659–663. doi: 10.1042/bj1710659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  16. Winsnes A. Studies on the activation in vitro of glucuronyltransferase. Biochim Biophys Acta. 1969 Nov 4;191(2):279–291. doi: 10.1016/0005-2744(69)90247-2. [DOI] [PubMed] [Google Scholar]
  17. Zakim D., Vessey D. A. Membrane-bound estrone as substrate for microsomal UDP-glucuronyltransferase. J Biol Chem. 1977 Nov 10;252(21):7534–7537. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES