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. 1980 Sep 1;189(3):475–480. doi: 10.1042/bj1890475

Phospholipid asymmetry in rough- and smooth-endoplasmic-reticulum membranes of untreated and phenobarbital-treated rat liver.

I C Bollen, J A Higgins
PMCID: PMC1162027  PMID: 7213341

Abstract

Phospholipase C was used as a probe for the distribution of phospholipids about the membrane of rough and smooth microsomal fractions from normal and phenobarbital-treated rat liver. All membranes exhibited an asymmetric distribution, with phosphatidylethanolamine and phosphatidylserine concentrated in the inner leaflet of the bilayer and phosphatidylcholine and sphingomyelin concentrated in the outer leaflet. The only phospholipid showing a significant difference in distribution between fractions was phosphatidylcholine, which was shifted towards the outer leaflet in the smooth microsomal fraction compared with the rough microsomal fraction, and towards the outer leaflet in both rough and smooth microsomal fractions from phenobarbital-treated liver compared with the same preparations from untreated rat liver. Apart from this small change, the asymmetric distribution of phospholipids was conserved in microsomal fractions which had proliferated in response to phenobarbital and in which the protein composition had changed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. CONNEY A. H., DAVISON C., GASTEL R., BURNS J. J. Adaptive increases in drug-metabolizing enzymes induced by phenobarbital and other drugs. J Pharmacol Exp Ther. 1960 Sep;130:1–8. [PubMed] [Google Scholar]
  3. Dallner G., Ernster L. Subfractionation and composition of microsomal membranes: a review. J Histochem Cytochem. 1968 Oct;16(10):611–632. doi: 10.1177/16.10.611. [DOI] [PubMed] [Google Scholar]
  4. Davison S. C., Wills E. D. Phospholipid synthesis in rat liver endoplasmic reticulum after the administration of phenobarbitone and 20-methylcholanthrene. Biochem J. 1974 Jul;142(1):19–26. doi: 10.1042/bj1420019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Depierre J. W., Dallner G. Structural aspects of the membrane of the endoplasmic reticulum. Biochim Biophys Acta. 1975 Dec 29;415(4):411–472. doi: 10.1016/0304-4157(75)90006-4. [DOI] [PubMed] [Google Scholar]
  6. Glaumann H., Dallner G. Lipid composition and turnover of rough and smooth microsomal membranes in rat liver. J Lipid Res. 1968 Nov;9(6):720–729. [PubMed] [Google Scholar]
  7. Higgins J. A. Asymmetry of the site of choline incorporation into phosphatidylcholine of rat liver microsomes. Biochim Biophys Acta. 1979 Nov 16;558(1):48–57. doi: 10.1016/0005-2736(79)90314-6. [DOI] [PubMed] [Google Scholar]
  8. Higgins J. A., Dawson R. M. Asymmetry of the phospholipid bilayer of rat liver endoplasmic reticulum. Biochim Biophys Acta. 1977 Nov 1;470(3):342–356. doi: 10.1016/0005-2736(77)90126-2. [DOI] [PubMed] [Google Scholar]
  9. Higgins J. A., Evans W. H. Transverse organization of phospholipids across the bilayer of plasma-membrane subfractions of rat hepatocytes. Biochem J. 1978 Aug 15;174(2):563–567. doi: 10.1042/bj1740563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Higgins J. A. Studies on the biogenesis of smooth endoplasmic reticulum membranes in hepatocytes of phenobarbital-treated rats. II. The site of phospholipid synthesis in the initial phase of membrane proliferation. J Cell Biol. 1974 Sep;62(3):635–646. doi: 10.1083/jcb.62.3.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Krebs J. J., Hauser H., Carafoli E. Asymmetric distribution of phospholipids in the inner membrane of beef heart mitochondria. J Biol Chem. 1979 Jun 25;254(12):5308–5316. [PubMed] [Google Scholar]
  12. Kreibich G., Debey P., Sabatini D. D. Selective release of content from microsomal vesicles without membrane disassembly. I. Permeability changes induced by low detergent concentrations. J Cell Biol. 1973 Aug;58(2):436–462. doi: 10.1083/jcb.58.2.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kreibich G., Ulrich B. L., Sabatini D. D. Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorins I and II, membrane proteins characteristics of rough microsomes. J Cell Biol. 1978 May;77(2):464–487. doi: 10.1083/jcb.77.2.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morimoto T., Matsuura S., Sasaki S., Yashiro Y., Omura T. Immunochemical and immunoelectron microscope studies on localization of NADPH-cytochrome c reductase on rat liver microsomes. J Cell Biol. 1976 Feb;68(2):189–201. doi: 10.1083/jcb.68.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nilsson O. S., Arion W. J., Depierre J. W., Dallner G., Ernster L. Evidence for the involvement of a glucose-6-phosphate carrier in microsomal glucose-6-phosphatase activity. Eur J Biochem. 1978 Jan 16;82(2):627–634. doi: 10.1111/j.1432-1033.1978.tb12059.x. [DOI] [PubMed] [Google Scholar]
  16. Nilsson O. S., Dallner G. Enzyme and phospholipid asymmetry in liver microsomal membranes. J Cell Biol. 1977 Mar;72(3):568–583. doi: 10.1083/jcb.72.3.568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Orrenius S., Ericsson J. L. Enzyme-membrane relationship in phenobarbital induction of synthesis of drug-metabolizing enzyme system and proliferation of endoplasmic membranes. J Cell Biol. 1966 Feb;28(2):181–198. doi: 10.1083/jcb.28.2.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Orrenius S., Ericsson J. L., Ernster L. Phenobarbital-induced synthesis of the microsomal drug-metabolizing enzyme system and its relationship to the proliferation of endoplasmic membranes. A morphological and biochemical study. J Cell Biol. 1965 Jun;25(3):627–639. doi: 10.1083/jcb.25.3.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. REMMER H., MERKER H. J. DRUG-INDUCED CHANGES IN THE LIVER ENDOPLASMIC RETICULUM: ASSOCIATION WITH DRUG-METABOLIZING ENZYMES. Science. 1963 Dec 27;142(3600):1657–1658. doi: 10.1126/science.142.3600.1657. [DOI] [PubMed] [Google Scholar]
  20. Redman C. M., Cherian M. G. The secretory pathways of rat serum glycoproteins and albumin. Localization of newly formed proteins within the endoplasmic reticulum. J Cell Biol. 1972 Feb;52(2):231–245. doi: 10.1083/jcb.52.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stier A., Sackmann E. Spin labels as enzyme substrates. Heterogeneous lipid distribution in liver microsomal membranes. Biochim Biophys Acta. 1973 Jul 6;311(3):400–408. doi: 10.1016/0005-2736(73)90320-9. [DOI] [PubMed] [Google Scholar]
  22. Stäubli W., Hess R., Weibel E. R. Correlated morphometric and biochemical studies on the liver cell. II. Effects of phenobarbital on rat hepatocytes. J Cell Biol. 1969 Jul;42(1):92–112. doi: 10.1083/jcb.42.1.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sundler R., Sarcione S. L., Alberts A. W., Vagelos P. R. Evidence against phospholipid asymmetry in intracellular membranes from liver. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3350–3354. doi: 10.1073/pnas.74.8.3350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weltzien H. U. Cytolytic and membrane-perturbing properties of lysophosphatidylcholine. Biochim Biophys Acta. 1979 Aug 20;559(2-3):259–287. doi: 10.1016/0304-4157(79)90004-2. [DOI] [PubMed] [Google Scholar]
  25. Young D. L., Powell G., McMillan W. O. Phenobarbital-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum. J Lipid Res. 1971 Jan;12(1):1–8. [PubMed] [Google Scholar]

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