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. 1980 Aug 15;190(2):461–464. doi: 10.1042/bj1900461

Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings.

K S Srivenugopal, P R Adiga
PMCID: PMC1162113  PMID: 7470060

Abstract

An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.

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Selected References

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