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. 1980 Oct 1;191(1):111–116. doi: 10.1042/bj1910111

Production of a variant of beta-lactamase II with selectively decreased cephalosporinase activity by a mutant of Bacillus cereus 569/H/9.

G S Baldwin, G F Edwards, P A Kiener, M J Tully, S G Waley, E P Abraham
PMCID: PMC1162187  PMID: 6781486

Abstract

1. Mutants of Bacillus cereus 569/H/9 have been screened in a search for strains that synthesize variants of beta-lactamase II. 2. One of these mutants (strain 569/H/9/1) produces a beta-lactamase II-like enzyme that shows a selective decrease in cephalosporinase activity. 3. beta-Lactamase II from strain 569/H/9/1 has been purified to apparent homogeneity and its kinetic properties have been examined. This enzyme resembles the parent beta-lactamase II in its relative activity with benzylpenicillin as substrate when Zn(II) is replaced by other metal ions, but differs detectably from the parent enzyme in its isoelectric point.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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