Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1980 Nov 1;191(2):509–516. doi: 10.1042/bj1910509

The purification and characterization of a third storage protein (convicilin) from the seeds of pea (Pisum sativum L.).

R R Croy, J A Gatehouse, M Tyler, D Boulter
PMCID: PMC1162241  PMID: 7236207

Abstract

A third storage protein, distinct from legumin and vicilin, has been purified from the seeds of pea (Pisum sativum L.). This protein has been named 'convicilin' and is present in protein bodies isolated from pea seeds. Convicilin has a subunit mol.wt. of 71 000 and a mol.wt. in its native form of 290 000. Convicilin is antigenically dissimilar to legumin, but gives a reaction of identity with vicilin when tested against antibodies raised against both proteins. However, convicilin contains no vicilin subunits and may be clearly separated from vicilin by non-dissociating techniques. Unlike vicilin, convicilin does not interact with concanavalin A, and contains insignificant amounts of carbohydrates. Limited heterogeneity, as shown by isoelectric focusing, N-terminal analysis, and CNBr cleavage, is present in convicilin isolated from a single pea variety; genetic variation of the protein between pea lines has also been observed.

Full text

PDF
509

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Casey R. Immunoaffinity chromatography as a means of purifying legumin from Pisum (pea) seeds. Biochem J. 1979 Feb 1;177(2):509–520. doi: 10.1042/bj1770509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HIRS C. H. The oxidation of ribonuclease with performic acid. J Biol Chem. 1956 Apr;219(2):611–621. [PubMed] [Google Scholar]
  3. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  4. Livingston D. M. Immunoaffinity chromatography of proteins. Methods Enzymol. 1974;34:723–731. doi: 10.1016/s0076-6879(74)34094-3. [DOI] [PubMed] [Google Scholar]
  5. Woods K. R., Wang K. T. Separation of dansyl-amino acids by polyamide layer chromatography. Biochim Biophys Acta. 1967 Feb 21;133(2):369–370. doi: 10.1016/0005-2795(67)90078-5. [DOI] [PubMed] [Google Scholar]
  6. Wright D. J., Boulter D. Purification and subunit structure of legumin of Vicia faba L. (broad bean). Biochem J. 1974 Aug;141(2):413–418. doi: 10.1042/bj1410413. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES