Abstract
Transport of D-fructose and D-galactose across the plasma membrane of isolated rat hepatocytes was followed for the net entry of sugars into sugar-free cells at 20 degrees C. Initial rates of transport showed a Michaelis-Menten dependency on sugar concentration, and transport was inhibited by 3-O-methyl-D-glucose in the external medium.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baur H., Heldt H. W. Transport of hexoses across the liver-cell membrane. Eur J Biochem. 1977 Apr 1;74(2):397–403. doi: 10.1111/j.1432-1033.1977.tb11404.x. [DOI] [PubMed] [Google Scholar]
- Berry M. N., Friend D. S. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969 Dec;43(3):506–520. doi: 10.1083/jcb.43.3.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Craik J. D., Elliott K. R. Kinetics of 3-O-methyl-D-glucose transport in isolated rat hepatocytes. Biochem J. 1979 Aug 15;182(2):503–508. doi: 10.1042/bj1820503. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goresky C. A., Bach G. G., Nadeau B. E. On the uptake of materials by the intact liver. The transport and net removal of galactose. J Clin Invest. 1973 May;52(5):991–1009. doi: 10.1172/JCI107300. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goresky C. A., Nadeau B. E. Uptake of materials by the intact liver. The exchange of glucose across the cell membranes. J Clin Invest. 1974 Feb;53(2):634–646. doi: 10.1172/JCI107598. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hooper R. H., Short A. H. The hepatocellular uptake of glucose, galactose and fructose in conscious sheep. J Physiol. 1977 Jan;264(2):523–539. doi: 10.1113/jphysiol.1977.sp011681. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keiding S., Johansen S., Winkler K., Tonnesen K., Tygstrup N. Michaelis-Menten kinetics of galactose elimination by the isolated perfused pig liver. Am J Physiol. 1976 May;230(5):1302–1313. doi: 10.1152/ajplegacy.1976.230.5.1302. [DOI] [PubMed] [Google Scholar]
- Sestoft L., Fleron P. Determination of the kinetic constants of fructose transport and phosphorylation in the perfused rat liver. Biochim Biophys Acta. 1974 Apr 12;345(1):27–38. doi: 10.1016/0005-2736(74)90242-9. [DOI] [PubMed] [Google Scholar]
- WILKINSON G. N. Statistical estimations in enzyme kinetics. Biochem J. 1961 Aug;80:324–332. doi: 10.1042/bj0800324. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woods H. F., Eggleston L. V., Krebs H. A. The cause of hepatic accumulation of fructose 1-phosphate on fructose loading. Biochem J. 1970 Sep;119(3):501–510. doi: 10.1042/bj1190501. [DOI] [PMC free article] [PubMed] [Google Scholar]