Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1980 Dec 15;192(3):947–950. doi: 10.1042/bj1920947

Ontogenic development of alpha-adrenergic receptors and responsiveness in white adipocytes.

R Pecquery, Y Giudicelli
PMCID: PMC1162422  PMID: 6263265

Abstract

The influence of age on adipocyte alpha-adrenergic receptors was studied by using the binding of [3H]dihydroergocryptine to crude adipocyte membranes from hamsters of different ages (1-10 months). The number of alpha-receptor sites was found to increase with increasing age, but in contrast, their binding affinity remained unchanged. These changes in receptor concentrations, which were not related to cell-size differences, were also accompanied by parallel variations of the alpha-adrenergic responsiveness of the fat-cells.

Full text

PDF
947

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avruch J., Wallach D. F. Preparation and properties of plasma membrane and endoplasmic reticulum fragments from isolated rat fat cells. Biochim Biophys Acta. 1971 Apr 13;233(2):334–347. doi: 10.1016/0005-2736(71)90331-2. [DOI] [PubMed] [Google Scholar]
  2. Berlan M., Dang Tran L. Intervention de récepteurs adrénergiques alpha et bêta dans l'effet des catécholamines sur la lipolyse d'adipocytes de chien. J Physiol (Paris) 1978 Dec;74(6):601–608. [PubMed] [Google Scholar]
  3. Borer K. T., Kelch R. P. A heterologous radioimmunoassay method for measurement of hamster growth hormone. Neuroendocrinology. 1978;25(2):65–76. doi: 10.1159/000122730. [DOI] [PubMed] [Google Scholar]
  4. Brown B. L., Albano J. D., Ekins R. P., Sgherzi A. M. A simple and sensitive saturation assay method for the measurement of adenosine 3':5'-cyclic monophosphate. Biochem J. 1971 Feb;121(3):561–562. doi: 10.1042/bj1210561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burns T. W., Langley P. E., Robison G. A. Adrenergic receptors and cyclic AMP in the regulation of human adipose tissue lipolysis. Ann N Y Acad Sci. 1971 Dec 30;185:115–128. doi: 10.1111/j.1749-6632.1971.tb45242.x. [DOI] [PubMed] [Google Scholar]
  6. Fain J. N. Biochemical aspects of drug and hormone action on adipose tissue. Pharmacol Rev. 1973 Mar;25(1):67–118. [PubMed] [Google Scholar]
  7. García-Sáinz J. A., Fain J. N. Effect of insulin, catecholamines and calcium ions on phospholipid metabolism in isolated white fat-cells. Biochem J. 1980 Mar 15;186(3):781–789. doi: 10.1042/bj1860781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Giudicelli Y., Agli B., Brulle D., Nordmann R. Influence of alpha-adrenergic blocking agents on cyclic AMP, cyclic GMP and lipolysis in hamster white fat cells. FEBS Lett. 1977 Nov 15;83(2):225–230. doi: 10.1016/0014-5793(77)81010-7. [DOI] [PubMed] [Google Scholar]
  9. Giudicelli Y., Lacasa D., Agli B. White fat cell alpha-adrenergic receptors and responsiveness in altered thyroid status. Biochem Biophys Res Commun. 1980 Jun 30;94(4):1113–1122. doi: 10.1016/0006-291x(80)90534-3. [DOI] [PubMed] [Google Scholar]
  10. Giudicelli Y., Pecquery R., Agli B., Nordmann R. Influences of cell size and aging on l-[3H] norepinephrine binding sites in rat epididymal fat cells. Biochimie. 1977;59(11-12):885–891. doi: 10.1016/s0300-9084(78)80703-2. [DOI] [PubMed] [Google Scholar]
  11. Giudicelli Y., Pecquery R. beta-Adrenergic receptors and catecholamine-sensitive adenylate cyclase in rat fat-cell membranes: influence of growth, cell size and aging. Eur J Biochem. 1978 Oct;90(2):413–419. doi: 10.1111/j.1432-1033.1978.tb12619.x. [DOI] [PubMed] [Google Scholar]
  12. Goldrick R. B., McLoughlin G. M. Lipolysis and lipogenesis from glucose in human fat cells of different sizes. Effects of insulin, epinephrine, and theophylline. J Clin Invest. 1970 Jun;49(6):1213–1223. doi: 10.1172/JCI106335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Herrmann J., Rusche H. J., Kröll H. J., Hilger P., Krüskemper H. L. Free triiodothyronine (t3)- and thyroxine (t4) serum levels in old age. Horm Metab Res. 1974 May;6(3):239–240. doi: 10.1055/s-0028-1095709. [DOI] [PubMed] [Google Scholar]
  14. Hittelman K. J., Wu C. F., Butcher R. W. Control of cyclic AMP levels in isolated fat cells from hamsters. Biochim Biophys Acta. 1973 Mar 30;304(1):188–196. doi: 10.1016/0304-4165(73)90127-x. [DOI] [PubMed] [Google Scholar]
  15. Lafontan M. Inhibition of epinephrine-induced lipolysis in isolated white adipocytes of aging rabbits by increased alpha-adrenergic responsiveness. J Lipid Res. 1979 Feb;20(2):208–216. [PubMed] [Google Scholar]
  16. Livingston J. N., Cuatrecasas P., Lockwood D. H. Studies of glucagon resistance in large rat adipocytes: 125I-labeled glucagon binding and lipolytic capacity. J Lipid Res. 1974 Jan;15(1):26–32. [PubMed] [Google Scholar]
  17. Nakano J., Gin A. C., Ishii T. Effect of age on norepinephrine-, ACTH-, theophylline- and dibutryl cyclic AMP-induced lipolysis in isolated rat fat cells. J Gerontol. 1971 Jan;26(1):8–12. doi: 10.1093/geronj/26.1.8. [DOI] [PubMed] [Google Scholar]
  18. Pecquery R., Giudicelli Y. Heterogeneity and subcellular localization of hamster adipocyte alpha-adrenergic receptors: evidence of alpha 1- and alpha 2-subtypes. FEBS Lett. 1980 Jul 11;116(1):85–90. doi: 10.1016/0014-5793(80)80534-5. [DOI] [PubMed] [Google Scholar]
  19. Pecquery R., Malagrida L., Giudicelli Y. Direct biochemical evidence for the existence of alpha-adrenergic receptors in hamster white adipocyte membranes. FEBS Lett. 1979 Feb 15;98(2):241–246. doi: 10.1016/0014-5793(79)80191-x. [DOI] [PubMed] [Google Scholar]
  20. Williams L. T., Mullikin D., Lefkowitz R. J. Identification of alpha-adrenergic receptors in uterine smooth muscle membranes by [3H]dihydroergocryptine binding. J Biol Chem. 1976 Nov 25;251(22):6915–6923. [PubMed] [Google Scholar]
  21. Zinder O., Shapiro B. Effect of cell size on epinephrine- and ACTH-induced fatty acid release from isolated fat cells. J Lipid Res. 1971 Jan;12(1):91–95. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES