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. 1980 Apr 15;188(1):61–65. doi: 10.1042/bj1880061

Dietary regulation of mammary lipogenesis in lactating rats.

M R Grigor, S M Warren
PMCID: PMC1162537  PMID: 7406889

Abstract

The proportion of medium-chain fatty acids (C8:0, C10:0 and C12:0) in rat milk increased significantly between day 4 and day 8 of lactation and for the remainder of lactation these acids comprised 40-50mol% of the total fatty acids. The milk fatty acid composition from day 8 was markedly dependent on the presence of dietary fat and altered to include the major fatty acids of the fats (peanut oil, coconut oil and linseed oil). The distribution of fatty acids made within the gland, however, was independent of dietary lipid and C8:0, C10:0 and C12:0 acids accounted for over 70% of the fatty acids made. The rates of lipogenesis in both the mammary gland and liver determined in vivo after the administration of 3H2O were affected by the presence of dietary lipid. In the mammary gland the rate for rats fed a diet containing peanut oil for 7 days was only one fifth that for rats fed a fat-free diet. Coconut oil also suppressed lipogenesis. Both dietary fats also suppressed lipogenesis in the liver.

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Selected References

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  1. BEARE J. L., GREGORY E. R., SMITH D. M., CAMPBELL J. A. The effect of rapeseed oil on reproduction and on the composition of ratmilkfat. Can J Biochem Physiol. 1961 Feb;39:195–201. doi: 10.1139/o61-016. [DOI] [PubMed] [Google Scholar]
  2. Bartley J. C., Abraham S. The absolute rate of fatty acid synthesis by mammary gland slices from lactating rats. J Lipid Res. 1976 Sep;17(5):467–477. [PubMed] [Google Scholar]
  3. Chivers L., Knudsen J., Dils R. Immunological properties of medium-chain acyl-thioester hydrolase and fatty acid synthetase from lactating-rabbit mammary gland. Biochim Biophys Acta. 1977 May 25;487(2):361–367. doi: 10.1016/0005-2760(77)90012-1. [DOI] [PubMed] [Google Scholar]
  4. Cooper S. M., Grigor M. R. Stereospecific biosynthesis of triacylglycerols in mammary glands from lactating rats. Biochem J. 1978 Aug 15;174(2):659–662. doi: 10.1042/bj1740659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cowie A. T. Variations in the yield and composition of the milk during lactation in the rabbit and the galactopoietic effect of prolactin. J Endocrinol. 1969 Jul;44(3):437–450. doi: 10.1677/joe.0.0440437. [DOI] [PubMed] [Google Scholar]
  6. Craig M. C., Nepokroeff C. M., Lakshmanan M. R., Porter J. W. Effect of dietary change on the rates of synthesis and degradation of rat liver fatty acid synthetase. Arch Biochem Biophys. 1972 Oct;152(2):619–630. doi: 10.1016/0003-9861(72)90258-5. [DOI] [PubMed] [Google Scholar]
  7. DILS R., POPJAK G. Biosynthesis of fatty acids in cell-free preparations. 5. Synthesis of fatty acids from acetate in extracts of lactating-rat mammary gland. Biochem J. 1962 Apr;83:41–51. doi: 10.1042/bj0830041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Demarne Y., Lhuillery C., Pihet J., Martinet L., Flanzy J. Comparative study of triacylglycerol fatty acids in milk from two Leporidae species: rabbit (Oryctolagus cuniculus) and hare (Lepus europaeus). Comp Biochem Physiol B. 1978;61(2):223–226. doi: 10.1016/0305-0491(78)90165-7. [DOI] [PubMed] [Google Scholar]
  9. Glass R. L., Troolin H. A., Jenness R. Comparative biochemical studies of milks. IV. Constituent fatty acids of milk fats. Comp Biochem Physiol. 1967 Aug;22(2):415–425. doi: 10.1016/0010-406x(67)90604-4. [DOI] [PubMed] [Google Scholar]
  10. Goulding A., Malthus R. S. Effect of dietary magnesium on the development of nephrocalcinosis in rats. J Nutr. 1969 Mar;97(3):353–358. doi: 10.1093/jn/97.3.353. [DOI] [PubMed] [Google Scholar]
  11. INSULL W., Jr, HIRSCH J., JAMES T., AHRENS E. H., Jr The fatty acids of human milk. II. Alterations produced by manipulation of caloric balance and exchange of dietary fats. J Clin Invest. 1959 Feb;38(2):443–450. doi: 10.1172/JCI103819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Knudsen J., Clark S., Dils R. Purification and some properties of a medium-chain acyl-thioester hydrolase from lactating-rabbit mammary gland which terminates chain elongation in fatty acid synthesis. Biochem J. 1976 Dec 15;160(3):683–691. doi: 10.1042/bj1600683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Libertini L. J., Smith S. Purification and properties of a thioesterase from lactating rat mammary gland which modifies the product specificity of fatty acid synthetase. J Biol Chem. 1978 Mar 10;253(5):1393–1401. [PubMed] [Google Scholar]
  14. Libertini L. J., Smith S. Synthesis of long chain acyl-enzyme thioesters by modified fatty acid synthetases and their hydrolysis by a mammary gland thioesterase. Arch Biochem Biophys. 1979 Jan;192(1):47–60. doi: 10.1016/0003-9861(79)90070-5. [DOI] [PubMed] [Google Scholar]
  15. McCullagh K. G., Lincoln H. G., Southgate D. A. Fatty acid composition of milk fat of the African elephant. Nature. 1969 May 3;222(5192):493–494. doi: 10.1038/222493a0. [DOI] [PubMed] [Google Scholar]
  16. Mellies M. J., Ishikawa T. T., Gartside P. S., Burton K., MacGee J., Allen K., Steiner P. M., Brady D., Glueck C. J. Effects of varying maternal dietary fatty acids in lactating women and their infants. Am J Clin Nutr. 1979 Feb;32(2):299–303. doi: 10.1093/ajcn/32.2.299. [DOI] [PubMed] [Google Scholar]
  17. Robinson A. M., Girard J. R., Williamson D. H. Evidence for a role of insulin in the regulation of lipogenesis in lactating rat mammary gland. Measurements of lipogenesis in vivo and plasma hormone concentrations in response to starvation and refeeding. Biochem J. 1978 Oct 15;176(1):343–346. doi: 10.1042/bj1760343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Romsos D. R., Leveille G. A. Effect of diet on activity of enzymes involved in fatty acid and cholesterol synthesis. Adv Lipid Res. 1974;12(0):97–146. [PubMed] [Google Scholar]
  19. Romsos D. R., Muiruri K. L., Lin P. Y., Leveille G. A. Influence of dietary fat, fasting, and acute premature weaning on in vivo rates of fatty acid synthesis in lactating mice. Proc Soc Exp Biol Med. 1978 Nov;159(2):308–312. doi: 10.3181/00379727-159-40338. [DOI] [PubMed] [Google Scholar]
  20. Smith S., Gagné H. T., Pitelka D. R., Abraham S. The effect of dietary fat on lipogenesis in mammary gland and liver from lactating and virgin mice. Biochem J. 1969 Dec;115(4):807–815. doi: 10.1042/bj1150807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Strong C. R., Dils R. Fatty acids synthesized by mammary gland slices from lactating guinea pig and rabbit. Comp Biochem Physiol B. 1972 Nov 15;43(3):643–652. doi: 10.1016/0305-0491(72)90149-6. [DOI] [PubMed] [Google Scholar]
  22. Tollerz G., Lindberg P. Influence of dietary fat and short-time starvation on the composition of sow-milk fat. Acta Vet Scand. 1965;6(2):118–134. doi: 10.1186/BF03547072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Volpe J. J., Lyles T. O., Roncari D. A., Vagelos P. R. Fatty acid synthetase of developing brain and liver. Content, synthesis, and degradation during development. J Biol Chem. 1973 Apr 10;248(7):2502–2513. [PubMed] [Google Scholar]

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