Abstract
A sub-line with increased metastatic ability was previously isolated from an established human colonic carcinoma cell line [Kimball & Brattain (1980) Cancer Res. 40, 1574-1579]. The separation and characterization of the isoenzymes of N-acetyl-beta-D-hexosaminidase from each cell line are reported. The parental cell line contained A and B isoenzymes. The sub-line lacked the A-isoenzyme activity and contained an atypical B isoenzyme that was thermolabile, susceptible to alkylation and of lower molecular weight.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bosmann H. B., Hall T. C. Enzyme activity in invasive tumors of human breast and colon. Proc Natl Acad Sci U S A. 1974 May;71(5):1833–1837. doi: 10.1073/pnas.71.5.1833. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brattain M. G., Green C., Kimball P. M., Marks M., Khaled M. Isoenzymes of beta-hexosaminidase from normal rat colon and colonic carcinoma. Cancer Res. 1979 Oct;39(10):4083–4090. [PubMed] [Google Scholar]
- Brattain M. G., Kimball P. M., Pretiow T. G., 2nd beta-Hexosaminidase isozymes in human colonic carcinoma. Cancer Res. 1977 Mar;37(3):731–735. [PubMed] [Google Scholar]
- Brattain M. G., Kimball P. M., Pretlow T. G., 2nd, Marks M. E. The interaction of N-acetylhexosaminidase with insolubilized concanavalin A. Biochem J. 1977 May 1;163(2):247–251. doi: 10.1042/bj1630247. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brattain M. G., Kimball P. M., Pretlow T. G., 2nd, Pitts A. M. Partial purification of human colonic carcinoma cells by sedimentation. Br J Cancer. 1977 Jun;35(6):850–857. doi: 10.1038/bjc.1977.128. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kimball P. M., Brattain M. G. Isolation of a cellular subpopulation from a human colonic carcinoma cell line. Cancer Res. 1980 May;40(5):1574–1579. [PubMed] [Google Scholar]
- Mian N., Cowen D. M. Glycosidases in normal and dimethylhydrazine-treated rats and mice with special reference to the colonic tumours. Br J Cancer. 1974 Jun;29(6):438–446. doi: 10.1038/bjc.1974.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mian N., Herries D. G., Batte E. A. The multiple forms and kinetic differences of rat colonic beta-N-acetylhexosaminidases. Biochim Biophys Acta. 1978 Apr 12;523(2):454–468. doi: 10.1016/0005-2744(78)90048-7. [DOI] [PubMed] [Google Scholar]
- Mian N., Herries D. G., Cowen D. M., Batte E. A. The multiple forms and kinetic properties of the N-acetyl-beta-D-hexosaminidases from colonic tumours and mucosa of rats treated with 1,2-dimethylhydrazine. Biochem J. 1979 Jan 1;177(1):319–330. doi: 10.1042/bj1770319. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Okochi T., Seike H., Higashino K., Hada T., Watanabe S., Yamamura Y., Ito F., Matsuda M., Osafune M., Kotake T. Alteration of hexosaminidase isozymes in human renal carcinoma. Cancer Res. 1979 May;39(5):1829–1834. [PubMed] [Google Scholar]
- Tsao D., Freeman H. J., Kim Y. S. beta-Hexosaminidase isoenzymes in tissues, cultured cells, and media from human fetal intestine and colonic adenocarcinoma. Cancer Res. 1979 Sep;39(9):3405–3410. [PubMed] [Google Scholar]
